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Wuji Solution regulatory role of H.pylori infection in mice stomach tissue protection and related cytokines

Author: PengCheng
Tutor: WuCanRong
School: Hunan University of Traditional Chinese Medicine
Course: Chinese and Western medicine combined with the basis
Keywords: Wuji Solution Helicobacter pylori C57BL / 6 mice Cytokines Apoptosis
CLC: R285.5
Type: Master's thesis
Year: 2006
Downloads: 11
Quote: 0
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Abstract


Objective: To investigate Dispensary Bureau Wuji Solution H · pylori infection of C57BL / 6 mice stomach tissue epithelial cells for the protection of mice infected gastric epithelial cell apoptosis in infected mice anti H.pylori The role; analysis Wuji Solution on mice infected serum IL-1β, IL-8, and IFN-γ were further Chinese medicine treatment of H · pylori infection and gastritis basis. METHODS: H · pylori infection of C57BL / 6 mice modeling, drug intervention after 7 days, take a mouse gastric tissue biopsy HE staining the rapid urine enzyme test, and stomach tissue homogenates poured blood agar plates were cultured, observed Wuji The protective effect of the drug in liquid H · pylori infection epithelial cells of the gastric tissue of mice mice inhibited and killed H · pylori's role; flow cytometry analysis of mouse stomach tissue mucosal epithelial cell apoptosis. Enzyme-linked immunosorbent assay (ELISA) detection Wuji Solution H · pylori infection in mice serum cytokines IL-1β, IL-8, and IFN-γ. Results: 1, treated mice The pathological changes in gastric mucosa with the model group mice gastric mucosa injury more significant difference (P lt; 0.05), Wuji Solution each dose could reduce the mouse stomach tissue mucosal lesions. Wuji Solution of each dose group and the model group mice stomach tissue H.pylori culture colony counts were significantly different (p lt; 0.05); Wuji Solution high dose group difference in the number of colonies of the clarithromycin group There was no significant (P gt; 0.05), to the Wuji Solution inhibited and killed the mice within the gastric tissue H.pylori.3, rapid urease test results show that: the significant difference between the treatment group and the model group (P lt; 0.05), with the increase of drug concentration, the rapid urease test negative rate is also increased. 4, the model mice gastric epithelial cell apoptosis rate than the normal group was significantly increased (P lt; 0.05); each treatment group, apoptosis rate was somewhat decreased (P lt; 0.05); Wuji Solution middle dose group and no significant difference compared clarithromycin group (P gt; 0.05). 5, model group, serum IL-1β content than normal group was significantly higher (P lt; 0.05); each treatment group, serum with the model group than the content of IL-1β (P lt; 0.05) were lower. Wuji Solution middle dose group, clarithromycin group with normal serum levels of IL-1β content compare the difference was not statistically significant significance (P gt; 0.05). 6, model group, serum IL-8 levels than normal group increased significantly (P lt; 0.05); each treatment group, serum IL-8 with the model group, were lower (P lt; 0.05). Wuji Solution for each dose group, no differences (P gt; 0.05) the clarithromycin group with normal mice of serum IL-8 content. Than the normal group, model group mice serum IFN-γ was significantly increased (P lt; 0.05); each treatment group, serum IFN-γ were compared with the model group were lower (P lt; 0.05); Wuji water Decoction dose group, the the clarithromycin group with normal mice serum IFN-γ content was no significant difference in sex (P gt; 0.05) Conclusion: 1, Wuji Solution in infected mice inhibited and killed H · pylori, to improve the the urease reaction negative rate, reduce the rate of apoptosis of gastric epithelial cells, better protect the gastric mucosa. Wuji Solution can reduce H pylori infection C57BL / 6 mouse serum IL-1β, IL-8, and IFN-γ content, alleviate the infected mice body inflammatory response.

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