|
This thesis work is derived from the national \Hop daughter Weijing soup, Ophiopogonis old TCM Mr. Zhou Zhongying found after long-term clinical practice experience in the treatment of lung cancer in side, classics side by Ophiopogon soup and daughter Weijing side two coincide. Ophiopogon soup seen in Zhongjing \Daughter Weijing party from Sun Ssu \The modern pharmacology studies confirm Ophiopogon soup and daughter Weijing square inhibit tumor growth and improve immunity. The subject is trying to provide the basis for research ideas and methods for the prescription of modern research to clarify the scientific connotation of lung cancer in the total treatment substance basic research through Ophiopogon Hop daughter Weijing soup effect. The first part of this thesis, the research of traditional Chinese medicine in the treatment of lung cancer were reviewed, analyzed and summarized and the aspects of the study of the chemical composition, pharmacological effects and clinical applications of Ophiopogon soup and daughter Weijing party. The second part of this thesis, the experiment content, first Ophiopogon Hop daughter Weijing soup water extraction, alcohol precipitation, extraction method eight chemical substances in parts (MW-of 01 ~~ MW-08), and using the MTT assay these parts of the biological effects of anti-lung cancer cell line A549 evaluation. Thereby determining a portion having a biological activity, respectively, for the ethyl acetate extract, and n-butanol parts, particularly the ethyl acetate extract best anti-lung effects. Silica gel column chromatography and recrystallization from ethyl acetate extract of the Hefang 11 chemical constituents were isolated by physicochemical properties and spectral analyzes of hydroxybenzoic acid, p-coumaric acid, fragrant oxalic acid, glycyrrhizin, different From both the n-butanol parts of succinic anhydride, uracil, ginsenoside Rg3 mannitol. Lung cancer cells in vitro anti-A549 Experimental results show that formononetin, uracil, ginsenoside Rg3, succinic acid, Iraq sitosterol inhibited the growth of A549 cells. UPLC-DAD-Q-TOF-MS technology, by reference substance than on UV absorption spectroscopy and mass spectrometry data, the final identification of the the nine chemical composition of the effect site, respectively, p-hydroxybenzoic acid, p-hydroxybenzaldehyde, vanillic acid, HPLC-DAD method for simultaneous determination of seven in the Ophiopogonis Hop daughter Weijing soup effect site the phenolic chemical composition (p-hydroxybenzoic acid, p-hydroxybenzaldehyde, p-coumaric acid, vanillic acid, glycyrrhizin, glycyrrhizin, different liquiritigenin). Chromatographic conditions for Waters sunfire C 18 (250mm x 4.6mm, 5um) column, the column temperature was 25 ° C, acetonitrile -0.5% acetic acid solution as mobile phase gradient elution, flow rate 1.0ml/min detection wavelength of hydroxybenzoic acid and liquiritin of 277.4nm. The rest of the compound detection wavelength 297.4nm. By column chromatography separation from the ethanol extract Weijing 14 compounds by 1H-NMR 13 C-NMR, 2D NMR and MS identified its structure: golden amide alcohol ester, ferulic acid, p-coumaric acid, syringic acid ,2,3-dihydroxy-1-(4-hydroxy-3 ,5-dimethoxyphenyl)-1-propanone, fragrant oxalic acid, hydroxybenzoic acid, p- formaldehyde, palmitic acid, heptadecanoic acid, β-sitosterol, stigmasterol, α-D-glucose and β-D-glucose. Evaluation of the biological activity of anti-lung cancer cell line A549, the monomer component obtained results showed that the growth inhibition of A549 cells role golden amide alcohol esters and ferulic acid are obvious. Features and innovations of this paper: chemical separation and pharmacological studies in close connection with the biological activity evaluation: First of all parts of the compound and its main Smell separation, determine the effective parts; modern chromatographic analysis techniques and effective parts get chemical ingredients, and their activity was evaluated, thereby estimating the active substance may be in the compound, to thereby provide the basis In order to clarify the effects of the therapeutic effect of the compound material basis. 2, a variety of advanced instrumental analysis methods used: a variety of modern chromatographic techniques for the analysis of complex chemical compound substances, in particular the UPLC-QTof-MS system with fully automated HPLC analysis detectable and multi-component quantitative analysis to provide a powerful and reliable technical support. 3, Ophiopogon Hop daughter Weijing soup is the old TCM Mr. Zhou Zhongying after long-term clinical practice found significantly the treatment of lung cancer, the basis of binding studies and biological effects of chemical substances through the paper, lay the foundation to create prevention and treatment of lung cancer Traditional Chinese Drug Research .
|