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The experimental duodenal nutrient perfusion the technology limiting amino acid sequence with the growing season goat microbial protein composition and digestive relationship. Liuyang Black Goat of muscle and microbial protein amino acids first analysis and evaluation, according to the production characteristics of Liuyang Black Goat established the small intestine can absorb the amino acid pattern target mode, while using the in vivo determination of the small intestine under basal diet conditions the liquidity and digestibility of various amino acids, the combination of the above two test results the dynamic incremental model established by amino acid amino acid perfusion. By duodenal perfusion declining-balance method based on application of different limiting amino acid in vivo determination of microbial protein and chyme amino acids liquidity and digestibility, thus proving the growing season goat limiting amino acid sequences, and come in limiting amino acid in corn - soybean meal diet conditions Liuyang Black Goat rumen microbial protein composition and absorption. This test is carried out in two phases. Determine the test of the first amino acid perfusion test restriction selected four good body condition, weighing about 10kg permanent rumen, proximal duodenum and terminal ileum fistula Liuyang Black Goat tested. Determination of Liuyang Black Goat gastrointestinal chyme of the different parts of the amino acids and microbial protein the liquidity and digestibility of amino acids under the conditions of the basal diet, muscle amino acid analysis data and goat growth characteristics to determine the specific amino acid perfusion dose. The test by using dynamic amino incremental model finalized lysine, methionine, leucine may be a first, second, third limiting amino acid, and its design for the second phase of the test perfusion amino. Phase II trial limiting amino acids of microbial protein amino acids and digesta amino acids trial period with 12 in good body condition, weighing about 10kg accordance with permanent rumen, the duodenum proximal and distal ileum fistula Liuyang Black Goat trials. Amino acid infusion in the duodenum, the amino acid infusion method for the reduction of perfusion, using a randomized block design, four pilot treatment groups I, II, Ⅲ, Ⅳ group were reductions perfusion group, lysine reduction infusion group, methionine reduction reperfusion group, leucine reduction perfusion group, collected in different parts of the chyme the chyme amino acids and microbial protein amino acids liquidity (or output), composition and digestibility. 2.1 amino acid infusion microbial protein in the rumen and duodenum, each test group the MCP production or liquidity differences are not significant (P> 0.05); in ileum parts, for this indicator, Ⅰ, Ⅱ, Ⅲ between the groups, the difference was not significant (P> 0.05), these three groups and Ⅳ differences were highly significant (P <0.01), in which the group Ⅳ ileum parts of the index. The largest proportion of essential amino acids and non-essential amino acids in the rumen part, Ⅰ group simultaneously Ⅰ, Ⅲ, Ⅳ group, the difference was not significant, three group II differences are significant; duodenum IV EAA / NEAA maximum ratio of I group III and group Ⅱ, Ⅳ significant difference; EAA / NEAA differences between the four groups in the ileum site not significant (P> 0.05). From the rumen to the duodenum at this stage, for the four amino acid R group is, MCP digestibility of about 10%, which fll the highest digestibility of, Iv group of the digestibility minimum, the difference was significant (P lt; 0.05). From the total digestibility of view, the the m groups digestibility minimum, and the other three groups was significantly different (P lt; 0 .05). Unit the chyme amino acids rumen parts 2.2 amino acid infusion organics chyme amino acids liquidity difference was not significant (P gt; 0.os); duodenum the the nl group and H, Iv group of units organics food Mi the amino acid liquidity differences significantly (P lt; 0 .05), where m group; the four perfusion group indexes were parts of the ileum significant cyanide P lt; 0.05). Rumen months of group I EAA, the EAA the maximum value, and and n, the Ifl Iv group, a significant difference (P lt; 0.05); duodenum, EA Where sigh EAA ratio difference significant (P lt; .05 ), wherein the maximum value of m group, n set of values ??is the smallest; minimum ratio in the ileum, maximum value of the group I EAAZNEAA, m group. From the rumen to the duodenum at this stage, the the chyme total amino acid digestibility for about 30% of the four amino acid infusion group difference was not significant (> 0.05). 75% 80% digestibility of amino acid chyme from the duodenum to the terminal ileum, where m group of amino acid digestibility of 75.32% of the lowest and the highest for the I n group 80.75%; For, EAA, His , Arg, Lys, ne, Leu, Phe digestibility in four amino acid infusion group difference was not significant (P gt; 0.05); the group I gamma, Met, Lys, Leu digestibility highest, ne digestibility minimum; n group Phe, val, the Arg digestibility highest; m group the His, Thr, val, Met, Lys digestibility level most Discovery Ile digestibility highest, in which Met digestibility and I group a significant difference (P lt; 0.05); w The group Tyr digestibility kick the other's, and three other group differences significant (P lt; 0,05). For the NEAA it, Asp, Glu, Gly, Cys digestibility differences are not significant (P gt; 0.05); the group I Ala, Pro digestibility highest, its Ala digestibility and m-group difference was significant (P lt; 0.05) Pro digestibility and the other three groups, the differences were significant (P lt; 0.05); the n group ser digestibility highest digestibility and I, nl group differences significant; nl group except Glu other NEAA digestibility of minimum: W group Gly to digestibility highest. The results showed that: (1) after duodenal perfusion different amino acid, will affect to the amino acid composition of the microbial protein; (2), methionine, lysine and leucine amino overall digestion different degree of influence of the absorption process; (3 ) under the conditions of the corn-soybean meal diet, methionine, lysine, leucine, histidine, arginine may be the limiting amino acids; the different amino acid (4) perfusion obvious effect on the digestibility of the MCP, impact order of methionine gt; lysine gt; leucine: (5) methionine chyme total amino acid digestibility more obvious than lysine and leucine.
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