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Objective: To use the one-dimensional hydrogen spectrum of nuclear magnetic resonance (1H nuclear magnetic resonance, 1H-NMR) technology stomach Mu point of functional dyspepsia (functional dyspepsia, FD) in rat plasma metabolites in. And the use of enzyme-linked immunosorbent assay (Enzyme-Linked Immunosorbent Assay, ELISA) to study gastric Mu point of the FD rat plasma gastrin levels. Methods: Male Wistar rats were randomly divided into five groups of 10 each. Respectively for the control group (control group), the model group (Model group), stomach Yu group (of Wei shu Model group), Zhongwan group (Zhong wan Model group), stomach Yu in Zhongwan group (Wei shu Zhong wan Model group). Electro-acupuncture stomach Yu hole, \. Spectra using 1H-NMR techniques to obtain plasma metabolites will crest normalized the numerical import one integrated software package SIMCA-P10.0 principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA), and combined orthogonal signal correction (OSC) method of data analysis, the corresponding score plot and factor loadings Figure. And rat plasma gastrin levels measured by ELISA. Results: 1, plasma gastrin levels measured results: 1) the model group less than the control group, a statistically significant difference between the two groups; 2) acupuncture group were higher than the model group, a statistically significant; 3) stomach Yu Zhongwan group than in the group of stomach Yu, Zhongwan group, a statistically significant relaxation time editing experimental results: 1) the model group and the control group was significantly separation, compared with the control rats, rat plasma model group in lactate (Lactate) content significantly decreased, acetone (Acetone) 3 - hydroxybutyrate (3-hydroxybutyrate) content there than the significantly increased; 2) stomach Yu in Zhongwan group and stomach Yu group, in the abdominal group three groups also presented than good separation, three groups stomach Yu zhongwan group, stomach the YU group, in the abdominal group compared with model group, acetone (Actone), 3 - hydroxybutyrate (3-hydroxybutyrate), choline / phosphatidylcholine (cho / pc) high density lipoprotein cholesterol / low-density lipoprotein (HDL / LDL) content decreased, and elevated levels of N-acetyl-glycoprotein (NAc); 3) stomach Yu of Zhongwan group stomach Yu group, Zhongwan group, the protein content of the acetylated sugar increasing trend. Conclusions: 1. Tail clamp stimulation catharsis function in rat liver, the spleen and stomach dysfunction caused by functional dyspepsia; 2.FD rat plasma gastrin levels decreased, EA stomach Yu Mu points can promote gastrin secretion, and stomach in the abdominal cavity can directly regulate Mu point has the joint, strengthen the role; stomach shu points adjustable liver catharsis function, promote secretion of bile excretion to adjust the stomach composting operation and features; stomach composting operation and features, can be used for the treatment of functional dyspepsia; 4. stomach Mu point both the effect of the two points, with obvious synergies through joint adjustment of the function of the stomach and hepatobiliary system, excellent efficacy in the single-cavity treatment; 6 in each group. relaxation time editing experimental techniques have emerged better or significantly separated, description of the experimental technique of metabolomics has played a good role in this experimental study, in this type of experimental study analysis in the use of a full feasibility worthy of further research.
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