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Allergic rhinitis Chinese dialectical element in vivo and cytokine research

Author: XiBin
Tutor: TianDaoFa
School: Hunan University of Traditional Chinese Medicine
Course: Integrative Medicine Otolaryngology
Keywords: Allergic rhinitis Trace elements Qi Deficiency Animal Model Guinea pig Cytokines
CLC: R276.1
Type: Master's thesis
Year: 2003
Downloads: 23
Quote: 0
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Abstract

Objective 1. Observe perennial allergic rhinitis (PAR) and hair serum levels of trace elements and their humoral immune function and Chinese medicine dialectical type correlation analysis of its pathological significance. (2) through the establishment of lung Deficiency Syndrome allergic rhinitis animal model was used to detect trace elements and serum levels of cytokines, cytokine network analysis Thl/Th2 imbalance for lung Deficiency AR pathological process impact, thereby Serum Trace element content and cytokine levels and lung qi type of allergic rhinitis pathological correlation process. Method 1 In clinical studies, the use of atomic absorption spectrophotometry PAR were detected in serum and hair elements Zn, Cu, Fe, Ca, Mg, Mn, Sr content, create an element discriminant model; turbidimetric assay PAR patients with immunoglobulin IgG, IgA, IgM, ELISA assay IgE. On the element values ??and correlation analysis of immunoglobulin levels to explore PAR three syndromes between trace element levels regularity. (2) In the experimental study, the use of smoked Act and TDI established by nasal stimulate allergic rhinitis in guinea pig lung Deficiency Syndrome combined model, atomic absorption spectrophotometry experimental allergic rhinitis guinea pig serum Zn, Cu, Ca content double antibody sandwich ELISA assay laboratory animal serum IFN-γ, IL-4 levels, and the element values ??and cytokine levels correlation analysis. Results 1 clinical study showed that three syndromes PAR serum and hair elements Mn, Sr lower than in the control group (P lt; 0.01), hair element Zn values ??decrease, Cu values ??increased, Zn / Cn ratio decreased (P lt; 0.05). Hair Sr content and IgM levels were negatively correlated with serum IgE levels Mn content was positively correlated with serum IgE and IgA Mg content was positively correlated. Both elements were established in serum and hair element discriminant model. 2 Experimental results show that guinea pig lung qi type of allergic rhinitis elevated levels of serum IL-4, IFN-γ levels decreased, IFN-γ/IL-4 ratio decreased. Compared with the healthy control group, the difference was significant (P lt; 0.01). Meanwhile, the value increased serum Zn (P lt; 0.05). Correlation analysis showed, Zn content and IL-4 levels were positively correlated with IFN-γ/IL-4 ratio was negatively correlated; Ca content and IL-4 levels were negatively correlated with IFN-γ/IL-4 ratio were positively correlated. Conclusion 1.PAR immune dysfunction in patients with serum and hair trace elements Zn, Cu, Mn, Sr change-related content, low Zn, low Mn, low Sr, Cu may be Benbingfasheng high intrinsic factors. 2.PAR basic pathogenesis of lung, spleen, kidney three dirty air yang deficiency. In the pathological process of development, and lesions from Yang Qi to gradually increase. Such deficiency in serum and hair are present Mn, Sr content decreased, suggesting that lung and spleen kidney yang deficiency may be associated with Mn, Sr content decreased with some relevance, particularly Mn values ??decrease, the incidence may be kidney-type PAR One of the important intrinsic mechanism. It was also found, Zn low, Cu high, Zn / Cu ratio decreased in differentiating Spleen, kidney deficiency has clinical significance. 3 According to PAR serum and hair trace element characteristics possessed randomness to explore the statistical regularity, according to Fischer guidelines established element discriminant mathematical model PAR mathematical diagnosis of a new idea. 4 Establish a lung Deficiency Syndrome allergic rhinitis animal model modeling method, for further in-depth study of PAR TCM purposes. 5.PAR disease itself and the performance of the clinical course of lung deficiency, were associated with Thl/Th2 cytokine network imbalance. Allergic rhinitis nasal mucosa showed to advantage Th2 immune response dominated allergic inflammation, lung deficiency is a cytokine network homeostasis homeostasis damage results. 6 In PAR pathological process, Zn, Ca elements may be involved in cytokine network Thl/Th2 balance adjustment mechanism. The findings suggest that loss through serum complement elements of PAR treatment may exert beneficial effects.

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