Dissertation > Excellent graduate degree dissertation topics show

Comparison of Genetic Diversity of Total Planktonic Bacteria and Active Planktonic Bacteria in Huguangyan Maar Lake in Summer

Author: GuoZuoRu
Tutor: WuZaoHe
School: Guangdong Ocean University
Course: Aquaculture
Keywords: Maar Lake(Hu Guangyan) Planktonic bacteria Active planktonic bacteria 16S rDNA ITS rpoB Vibrio Genetic diversity Phylogenetic analysis
CLC: Q938
Type: Master's thesis
Year: 2011
Downloads: 6
Quote: 0
Read: Download Dissertation


Planktonic bacteria are the most abundant microbes and widely distributed in aquatic ecosystem. They participate in material and energy metabolism and thus play a significant role in aquatic ecosystem. Hu Guangyan Maar Lake is a special kind of Crater Lake with old geological ages that makes it different from other lakes. Therefore it is valuable to research and develop microbial resources in Maar Lake. We investigated the genetic diversity of planktonic bacterial and active planktonic bacterial through constructing seven clone libraries of common lake microbial groups. Meanwhile, we used culture-depend method to study the genetic diversity of Vibrio.We chose one of the deepest sites and gather samples from three water layers (1 m, 5 m, and 13 m). Then we employed universal primer to amplify 16S rDNA from total DNA and total RNA from planktonic bacteria, respectively. Positive clones were chosen for sequencing. RDP analysis showed that all 414 sequences were classified into 15 phylums, 32 orders and 52 families. Four kinds of Proteobacteria (α-、β-、δ-、γ-Proteobacteria) were found in Maar Lake. Nearly half of the sequences were affiliated with Alphaproteobacteria, which was the most abundant group followed by Verrucomicrobia and Bacteroidetes. The depth might cause diversity discrepancy of planktonic bacteria. The diversity of planktonic bacteria in surface (1 m) seemed higher than bottom (10 m). The diversity of total planktonic bacteria is slightly higher than that of active planktonic bacterial in surface. The diversity of active planktonic bacterial is obviously higer than total planktonic bacteria in 10m. We got a clone of the OD1and Spirochaetes sequence that is the first time to be reported in domestic and foreign research of lake diversity.Seven universal primers were used to amplify 16S rDNA from the same environmental samples in order to analyze the difference of diversity in group levels. We constructed seven clone libraries of common microbial groups of lakes: Planctomycetes, Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria, Bacteroidetes, Firmicutes, and Actinobacteria. The results showed that the diversity of planktonic bacteria was different within different libraries and depths.Finally, we isolated vibrio. strains from Maar Lake by culture-depended method and compared them with those from Zhanjiang port nearby. By analysis of the sequences of ITS, most sequences (18 in 19) from port of Zhanjiang were affiliated to Vibrio while no sequences belonged to Vibrio. in isolates from the Maar Lake. All sequences amplified with rpoB gene suggested that the bacterial selected by TCBS from Zhanjiang port were all Vibrio. The use of ITS and rpoB gene as molecular markers for classification and identification of bacteria were basically the same in genus level but notable differences in species level.

Related Dissertations

  1. Comparison on Immunoprotective Effect of Bacteriophages of Vibrio Alginolyticus and Vaccines of Bacterial Ghost,S945
  2. Study on Phylogenetic Diversity and Bioactivity of Symbiotic Fungi of Coral,R284
  3. Genetic Diversity Analyses of Sillago Sihama Using AFLP Marker,S917.4
  4. Growth Rate and Growth Model of the F5 Selected Lines for Four Shell Colors of Pinctada Martensii (Dunker) Cultured in Deepwater Area of Beibu Bay in Zhanjiang and Their Genetic Diversity Analysis Using SSR Molecular Markers,S968.31
  5. Pathogenic Differentiation and Genetic Diversity of Wheat Sharp Eyespot in Henan Province,S435.121
  6. ISSR Analysis of Genetic Diversity on 21 Lotus(Nelumbo Nucifera) Cultivars,S682.32
  7. The Detection of the Virulence-related Gene from Vibrio Alginolyticus and the Study on Preservation Methods,S943
  8. Establishment of the Molecular Identifying System of Curvualria and Application in Difficult Species,Q949.32
  9. Genetic Diversity and Ultrastructural Studies of Southern Corn Rust in China,S435.131.4
  10. SRAP and SSR molecular markers of pink flower Dendrobium genetic diversity and population genetic structure,S567.239
  11. The Research of Microsatellite Markers and the Eatablishment of Growth and Development Model in Xianan Cattle and Pinan Cattle,S823
  12. Construction and Ests Analysis of the Subtraction Cdna Library for Larvae of Lutjanus Sanguineus Induced by Vibrio Alginolyticus,S943
  13. Proteomic Analysis of Vibrio Alginolyticus in Response to Four Types of Antibiotics,S941
  14. Screen and Functional Analysis of the Genes Regulate Aphb in Vibrio Cholerae and Inhibitory Activity of Pseudomonas Aeruginosa on Vibrio Cholerae,R378
  15. Transcriptional Regulation of Genes Encoding MFS by LysR in Vibrio Cholerae,R378
  16. Identification and Lipopeptide Analysis of Biocontrol Bacillus Spp. Isolated from Tibet,S476.1
  17. Identification、Diversity and Phylogenetic Analysis of Antagonistic Bacillus Isolated from Different Chinese Areas,S476.1
  18. Molecular Characterisation Based on ITS and Genetic Diversity of the Heterodera Avenae Group on the Huang-huai Floodplain of China,S435.121
  19. Characterization of Population Structure and Linkage Disequilibrium of Chinese Soybean Landerace Population and QTL Association Analysis of Traits Related to Breeding for Soybeans,S565.1
  20. Fine Mapping of Inter-Subspecies Hybrid Pollen Sterility Gene in Rice (Oryza Sativa L.) and Phylogenetic Analysis,S511

CLC: > Biological Sciences > Microbiology > Microbial ecology and geographical distribution
© 2012 www.DissertationTopic.Net  Mobile