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The Phylogenetics Relationships of the Genus Paramecium Inferred from Small Subunit rRNA Gene Sequences and Comparison of Morphology in P.bursaria-Chlorella System

Author: QianYu
Tutor: GuFuKang
School: East China Normal University
Course: Zoology
Keywords: Paramecium SSUrRNA gene Phylogenetic Silver Line system Symbiosis FLUTAX- paclitaxel Scanning electron microscopy
CLC: Q958.1
Type: Master's thesis
Year: 2005
Downloads: 110
Quote: 2
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Abstract


The papers containing symbiotic Chlorella Paramecium bursaria and symbiotic Chlorella green paramecium as experimental materials, application FLUTAX-paclitaxel staining and scanning electron microscopy, comparative study of two different state the green paramecium cell surface silver distribution line system: three groups within the genus paramecium the 13 kinds paramecium research materials, their 16s Small Subunit ribosomal RNA (16s-like the Small Subunit rRNA SSUrRNA) gene sequencing to Tetrahymena thermophila (Tetrahymena thermophila) as outgroup by CLUSTALW and MEGA2.0, all kinds of SSUrRNA sequence phylogenetic analysis to explore the evolution of the various classes of Paramecium genus system, especially those containing symbiotic ball green algae paramecium and non-symbiotic chlorella green paramecium SSUrRNA gene molecular genetic characteristics. 1 light, dark Paramecium bursaria culture conditions - Chlorella symbiotic system morphological study through the FLUTAX-paclitaxel staining, studies have shown that many symbiotic Chlorella located in the rear of the worm, and symbiotic algae how much with the host paramecium own nutritional status varies symbiotic algae disappear generally start parasites the front started to disappear from front to back; the symbiotic chlorella are located at the cell poles in the parasites mitosis. The authors speculate, the symbiotic Chlorella is not entirely random in host Green paramecium body, but cell polarity. Scanning electron microscopy study results found that the average size of the mouth before light insects silver line system grid is about: long 2.37um, width 1.5um; cilia 1.6um vertical spacing between the horizontal spacing of 1.5um. The dark insects silver wire system grid average size is about: length 1.47um wide 0.84um: cilia between longitudinal spacing 1.46um, horizontal spacing 1.05um. Mouth after light insects silver line system grid average size is about: long 1.8um, width 0.9um; the cilia vertical spacing 1.48um horizontal spacing 0.9um. The dark insects silver wire system grid average size is about: length 1.6um wide 0.7um; cilia vertical spacing 1.8um, horizontal pitch 1.8um. The back light the insects back of the silver line system grid average size is about: length 4.75um wide 4.2um; the cilia vertical spacing 4.6um horizontal spacing 3.6um. Dark insect surface silver line system grid average size is about: length 3.75um wide 3.3um: the cilia vertical spacing 4um cilia horizontal spacing 3um. Shows that contain symbiotic algae green paramecium cell volume than the surface of silver wire mesh size is slightly larger than the large the green paramecium cell volume symbiotic algae outside without symbiotic algae green paramecium, and silver wire system than without by symbiotic algae green paramecium significantly; without symbiotic algae green the paramecium surface mesh thickness compared with symbiotic algae green paramecium thick for. The results suggest that changes in cell morphology symbiotic algae green paramecium and within long-term mutual adaptation, and may thus affect the paramecium cilia movement. 2 the paramecium case within phylogenetic study was measured the Paramecium genus within 13 kinds of the paramecium SSUrRNA gene sequence, right, Kimura two-parameter method its genes SSUrRNA the base, the base position of codon genetic distance and standard error, and the conversion / transversion number and percentage of comparative analysis;, MP law and ML constructed a phylogenetic tree by the NJ method, the results reveal: (1) containing the symbiotic chlorella green paramecium and by change

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CLC: > Biological Sciences > Zoology > Animal ecology and zoogeography > Animal ecology
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