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In this study, PK-15 cells were isolated and cultured swine fever virus (CSFV) Wuzhou strain (GXWZ02) mice were immunized with purified establishment of McAb hybridoma secreting anti- CSFV strains . The strain passaged through five generations can better adapt passaged cell growth, measured by cytotoxic TCID 50 of 10 6 / ml. With purified cytotoxic mice were immunized by three significant effect after the third immunization , immunization of BALB / C mice hog cholera serum antibody titer reached 1:32000 . Spleen cells from the immunized mice with SP2 / 0 myeloma cell fusion of conventional methods , has conducted two fusion , a total of 660 fusion pore , screened by indirect ELISA , fusion -positive hole total of 468 holes , selected 145 positive integration limiting dilution holes cloned three times , won the 19 anti- CSFV monoclonal antibody secreting McAb hybridoma cell lines , ultra- low temperature at -80 ℃ for 3 months after the recovery is still stable hybridoma secreting anti- CSFV was McAb. Select from 10 Preparation of hybridoma ascites, indirect ELISA test for detection of the selected 10 ascites monoclonal antibody titer 1:400-1:6400 titer highest 1B3, 2C6 have reached 1:6400 , followed 2B5, 2A6 , 1F4, 1F8, 1B7 titers were reached : 1:3200,1 C7, 2B3 titer of 1:800 , the lowest 1A9 titer reached only 1:200 . Immunofluorescence assay (IFA) for the specific detection of McAb results show that these monoclonal antibodies from 1:50-1:400 diluted and inoculated GXWZ02 toxic cells can produce specific fluorescence reaction , each monoclonal antibody dilution : 2C6 is a : 400,1 F4, 1F8, 2A6, 2B5, 1B3, 1B7 is 1:200,1 C7, 2B3 to 1:100,1 A9 1:50. ELISA and IF qualification tests identified as anti- GXWZ02 monoclonal antibody , which
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