Dissertation > Excellent graduate degree dissertation topics show

Dioxin regulation of human endometrial cells in vitro expression of IL-6 and TNF-α studies

Author: FangQingXian
Tutor: LinJun
School: Zhejiang University
Course: Obstetrics and Gynaecology
Keywords: Dioxins Endometriosis IL-6 TNF-α Endometrial cells Pathogenesis
CLC: R711.71
Type: Master's thesis
Year: 2005
Downloads: 116
Quote: 0
Read: Download Dissertation

Abstract


Background Endometriosis (Endometriosis, EMs) increasingly rising incidence has become a serious impact on women's health and fertility of the common one, but so far the disease pathogenesis is not clear. Recent studies have found that environmental toxicants two (mouth evil) English EMs morbidity associated with the current environmental epidemiology and animal experiments from the study found that exposure to environmental toxins two (mouth evil) British individuals and animals, their EMs significantly higher incidence of However, environmental toxins two (mouth evil) English How To EMs disease, its mechanism is still unclear. EMs as a multifactorial pathogenesis of the disease, currently speculated two (mouth evil) English EMs may be induced by a variety of pathways in the pathogenesis of immune mechanisms through which is currently a hot research and focus. As the immune system cells mediated signal transduction factors, cytokines are considered to pathophysiological changes in EMs plays a key role, and two (mouth evil) England and the immune system itself is an important regulator of cytokine. IL-6 and TNF-α is a more recent study two cytokines associated with EMs, which is considered to EMs dysfunction disease closely related to the animal in vivo and in vitro findings bis (bad mouth) Inco regulate IL-6, TNF-α and other cytokines, so we speculate in the body two (mouth evil) England may regulate IL-6, TNF-α in the occurrence and development of EMs. In this study, human endometrial cells in primary culture, based on the mRNA and protein levels were measured from two (mouth evil) England would not affect IL-6, TNF-α secretion, and further compare EMs and endometrium of patients with non-EMs for two (mouth evil) English-induced allergic reactions are different, so as to explore two (mouth evil) English is by regulating IL-6 and TNF-Zhejiang University, a master's degree thesis expression in the pathogenesis of EMs in order to participate in the two ah English EMs onset further provide experimental evidence and explore its possible target molecules and mechanisms. Materials and Methods EMS eutopic endometrium from surgery in our hospital and histopathological diagnosis of ovarian endometriosis patients (n 2:13), the control group from the same period of endometrial surgical and histopathological confirmed non-EMS patients with uterine fibroids (n = 15). Endometrial cells in vitro culture, based on the first immunohistochemical S a P two-step identification of the cultured endometrial cells, and then MTT assay at different concentrations TCDD (0,0. InM, InM, 10nM, IOOnM) role in endometrial cells 24 hours on its activity, and then use the control group did endometrial cells dose (n = 4), respectively. , 0.olnM, 0.xnM, InM, xonM, loon. Hundred concentrations of TCDD action endometrial cells after 24 hours by real one t ime PCR method for the determination and the EL Engineering SA Engineering L-6 and TNF a Q mRNA and protein expression, on this basis, further reference to the role of literature were selected IOnMTCDD control group and the EMS group endometrial cells 24 hours, the same real one t ime PCR and ELISA, IL-6 and TNF ~ a mRNA and protein expression . With One one way ANOVA, Kruskal an WalliSH, t test, Mann a WhitneyU and WilcoxC, n test statistical methods of data between multiple groups and two sets of comparative analysis, test level Q = 0.05, bilateral. All experimental data SPSS10.O statistical package for processing. Results 1.IOnMTCDD effect after 24 hours can induce cultured human endometrial cells express higher levels of TNF-alpha. Protein, and the group of patients with EMS-induced effect is greater (P lt; 0 .05). 2.10nMTCDD for 24 hours after the inhibit endometrial cells work EMs group L-6 protein expression (P lt; 0.05), but no significant change in the control group (P gt; 0 .05). 3.IOnMTCDD effect 24 hours after cultured human endometrial cells in IL-6 and TNF-a mRNA expression did not change significantly (P gt; 0.05).

Related Dissertations

  1. Inhibition of RAW264.7 Macrophage Inflammatory Cytokines Release by Guggulsterone,R285
  2. Infectious Bursal Disease Virus Particle Vaccine Research and Its Immune Efficacy Evaluation,S858.31
  3. Roles of MMP-7 and Lysozyme in the Pathogenesis of Ulcerative Colitis Induced by DSS in Balb/c Mice,S858.91
  4. Preparation of Monoclonal Antibody to Porcine TNF-α and Expression of TNF-α in PAM Infected with PRRSV,S858.28
  5. The Expression and Significance of Nuclear Factor Kappa B (NF-κb) and IL-6 in the Degenerated Cervical Intervertebral Disc,R681.55
  6. Cysteamine on T Lymphocyte Proliferation in Post-Operation Goats and Its Mechanism Underlying,S858.27
  7. Cloning, Expression and Bioactivity Analysis of Goat Interleukin-1β and Interleukin-6,S827
  8. Meridians of Asthma Thread Burying Model Rats IL-4 Such Impact Experimental Study,R245
  9. Role of G31P in Smooth Muscle Migration of A7r5 Cells Induced by Interleukin-8,R363
  10. The Expression and Mechanism of NF-κ B and IL-6 after 90% Portal Vein Ligation in Rats,R657.3
  11. Malignant Transformation of Ovarian Endometriosis-related Protein Expression in the Meta-Analysis,R711.71
  12. The Sequence Analysis of RNA Components from Lactobacillus Metabolites and Parts of the Biological Function,R378
  13. Noble Dendrobium Polysaccharides Attenuate Learning and Memory Deficits Induced by Lipopolysaccharide and Its Mechanisms in Rats,R285.5
  14. Effect of Grape Seed Procyanidins on the Blood Pressure in Renovascular Hypertensive Rats and Its Mechanism,R544.1
  15. Experimental Study on 420nm Intense Pulse Light in Animal Acne Model,R758.733
  16. Biological Effects of Soluble CD40 Ligand on Leukemia HL-60 Cells and Its Mechanism,R733.7
  17. Cloning and Expression Analysis of NCCRP-1 AND IL-10 Genes in Grass Carp (Ctenopharyngodon Idellus),S917.4
  18. Clinical Observation of Ditan Soup in Treating PSD,R277.7
  19. Exploreing the Mechanism of Xieshifeiaifang Ii on Treating Lung Cancer with Pleura Effusion,R734.2
  20. Study on the Correlation Between the Levels of Il-18, IL-33 and Other Inflammatory Factors and Pulmonary Function in Different Stages of Asthma,R562.25
  21. Effects of Blockade of IL-17 in Already Established Asthma of BALB/c Mice,R562.25

CLC: > Medicine, health > Obstetrics and Gynaecology > Gynecology > Other diseases of the female genital > Endometriosis
© 2012 www.DissertationTopic.Net  Mobile