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Screening and Studying on Antioxidant Ability of B.breve A04 Toleranced to Gastrointestinal Tract

Author: JiangZhiJie
Tutor: LiPingLan
School: China Agricultural University
Course: Of Food Science
Keywords: Bifidobacterium Filter Digestive tract Radicals Anti-aging
CLC: R37
Type: Master's thesis
Year: 2005
Downloads: 148
Quote: 1
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Abstract


Bifidobacterium (Bifidobacterium) is an important physiological bacteria in the gut of humans and animals, its presence direct impact on the health status of the digestive tract. Currently, in our country and the world as a whole range of excellent Bifidobacterium resources development and utilization has become a hot spot. The subject by the method of in vitro and in vivo experiments, a total of five experiments, including the inverse-resistant gastrointestinal environment Bifidobacterium screening, growth characteristics and growth factors as well as the study of the antioxidant activity. (1) derived from the breast milk baby stool unweaned piglets faeces, commercially Bifidobacterium and stored in our laboratory bifidobacteria, using a modified MRS medium as the original strain, simulation of the human gastrointestinal tract inverse environment, its resistant gastrointestinal characteristics were studied. The research results show that the capacity of the A03, A04 and L-1 strain tolerance gastrointestinal reverse environmental best used as probiotics strains. Meanwhile, the growth rate of the A04 strain faster, therefore the final selection of the A04 strain by the preliminary identification of bifidobacteria A04 the Bifidobacterium breve named as Bifidobacterium breve A04. (2) using a modified MRS medium to study the growth characteristics of B.breve A04, the results showed that its optimum growth conditions: 35 ℃ -37 ℃, starting pH 7.0, inoculum size 3%, the highest number of viable cells Ig value reached 9.26, fast growing, acid-producing ability, the final pH of 3.78, acid resistance, relatively short lag phase for 2 h after the logarithmic growth phase, 10 h to reach stable period. (3) study the proliferation of various factors on the B.breve A04. The results show that the proliferative effect of the soybean oligosaccharides most significant (P lt; 0.01), followed Isomaltooligosaccharide and oligofructose, stachyose virtually no proliferative effect, but when the concentration of oligosaccharides in the MRS reach 10 %, its but has a certain inhibition; eight kinds of medicine in the concentration of 2% when both proliferative effect, to Z 3 , Z 1 and Z < sub> 2 effect the most significant (P lt; 0.01), but its concentration on the very different impact of the proliferation of B.breve A04; growth-promoting effect of four kinds of food materials have B.breve A04, better a combination of proliferative effects were: M-5%, CJ-2%, TE-2% and LE-5%. (4) in vitro free radical scavenging Preliminary experimental results found live bacteria culture supernatant and bacterial crushed materials have significant anti-lipid peroxidation ability to effectively scavenge DPPH and reactive oxygen free radicals, also protect DNA from free radical damage. (5) the use of subacute aging model mice D-galactose induced established research B.breve A04 live bacteria culture supernatant was disrupted bacterial anti-aging effect. Found that live bacteria culture supernatant and bacterial the crushed materials could reduce the content of MDA in serum and liver; able to increase serum SOD, CAT vitality and total antioxidant capacity; significantly reduce brain tissue lipofuscin and MAO content, can enhance the protein synthesis capability of the liver and brain tissue. The results showed that, B.breve A04 live bacteria culture supernatant and bacterial crushed materials with the role of aging, the mechanism may be achieved through a variety of channels.

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CLC: > Medicine, health > Basic Medical > Medical Microbiology ( pathogenic bacteriology,pathogenic microbiology )
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