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The Development and Preliminary Application of Multiple Antigenic Peptide Microarray Plateform

Author: YangHao
Tutor: YanXiaoJun;HanFengChan;ZhangHuMing
School: Fourth Military Medical University
Course: Biochemistry and Molecular Biology
Keywords: Multi - antigen peptide Peptide Synthesis B-cell epitope Microarray
CLC: Q503
Type: Master's thesis
Year: 2005
Downloads: 47
Quote: 0
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Abstract


Objective: Since the implementation and progress of the Human Genome Project , proteomics has become another hot spot Following the genomics field of life sciences . Around the world face a broad array of biological information , researchers developed a nucleic acid and protein microarray technology for high-throughput parallel analysis of these massive amounts of information , this revolutionary breakthrough in the traditional analysis methods in biological markers in screening , the interaction between the biomolecule , the functional study of biological molecules , as well as disease diagnosis, prognosis , has become one of the important techniques . Multiple antigen peptide microarray is another technological innovation after following nucleic acid microarrays and protein microarrays , which combines the advantages of the microarray analysis system in parallel , high-throughput , automated , and low application cost , will be analyzed objects point to the active sequence in the target protein macromolecules , and the modern proteomics technology into a deeper level . The purpose of the subject polypeptides multiple antigenic peptide - based design and synthesis technology to create a fast, high-throughput , automated , and low-cost microarray analysis platform for the identification of pathogenic microorganisms , typing , screening for pathogenic microorganisms vaccine -assisted design . Method : the basis for the work of the past , we have selected the human cytomegalovirus and Helicobacter pylori as the object of study of human cytomegalovirus envelope glycoprotein B and envelope alkaline phosphatase protein PP150

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