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Construction of Somatic Cell Bank and Clone of GPAT Gene of Beijing Oil Chicken

Author: ZhangHaiYan
Tutor: YueWenBin；GuanWeiJun
School: Shanxi Agricultural University
Course: Animal Genetic Breeding and Reproduction
Keywords: Beijing Oil chicken Somatic cell bank GPAT gene Clone
CLC: S831
Type: Master's thesis
Year: 2004
Downloads: 53
Quote: 0
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Abstract


Beijing Oil chicken that are famous for their flavor and the fine qualities of egg are one of precious poultry breed and endangered breed.In this study, Beijing Oil chicken embryos(9-13 days) were cultured with direct-adherent-cultivation methods and constructed successfully fibroblast somatic cells bank, the cell freezing density is between 3×10~6cell/ml and 5×10~6cell/ml. Biological analysis of cell bank showed that the growth curves appeared “S”shape,and illustrated that the cells growth have three phases that are latent phase,logarithmic mulplication phase and levels static phase;and that the cell survival rate surpassed 90% after cryopreservation;and that the analyses of the chromosomal karyotypes indicated that the cells were diploid, the hereditary character is stable;The results of cloning and sequencing showed the cloned GPAT gene fragment that coded Glutamine phosphoribosylpyrophosphate amidotransferase was 953 bp.The comparison of these code sequences with those of avian through blastn of GenBank,the results indicated that the similarity is 100%.That proved that the hereditary character of cultured cells is stable. Fibroblast somatic cells bank of Beijing oil chicken were constructed successfully,the method of construced cell bank were studied,and GPAT gene were cloned and analysised in this study ,The aim of this study was conservation Beijing Oil chicken genetic resources by somatic cells cryopreservation method,at the time, laying the foundation for the studying of factors influencing inosine monphosphate (IMP) concentration in genetic.

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CLC: > Agricultural Sciences > Livestock, animal medicine,hunting,silkworm,bee > Poultry > Chicken
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