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Objective: To investigate DNA topoisomerase II (topoisomerase II, Topo II) and specialized protein 1 (Specificity protein-1, Sp1) cisplatin (cisplatin, CDDP) for teniposide (tineposide, VM-26) anti- small cell lung cancer (small cell lung cancer, SCLC) cells affect the expression of the process . Methods: H446 cells ( human SCLC cell lines ) at 37 ℃, 5% CO2, saturated humidity, containing 10 % fetal bovine serum RPMI 1640 under subcultured cells in logarithmic growth phase until after the experimental operation . MTT assay , peak plasma concentrations were measured CDDP with different concentrations of VM-26 combination on the H446 cells 24 hours inhibition rate , the application of the Guinness Book of formula combined effect of drugs ; using AO / EB double fluorescent staining cell motility: the preparation of acridine orange (AO) , and olfactory ethidium (EB) of the 0.05 mg / ml of the dye mixture . Plus fluorescent dye in the cell suspension , the percussion mix, drops to a glass slide , fluorescence microscope , camera , cell viability were calculated ; using Hoechst 33258 fluorescent staining apoptosis rate : drugs H446 cells 24 hours after , EDTA digestion, made ??of 1 × 105 / ml single cell suspension , the methanol: acetic acid (3:1) 4 ℃ fixed for 5 minutes and added dropwise to a slide , dried , add dye Hoechst 33258 for 30 minutes, distilled water piece remove excess liquid , sealed tablets were mounted , set fluorescence microscope , camera ; using agarose gel electrophoresis DNA breaks : H446 cells were treated with drugs for 24 hours after the cells were collected and washed twice with saline , were added to cell lysate , 4 ℃ cracking 30 minutes ; adding RNA enzyme (50μg/ml) 37 ℃ 1 hour proteinase K, 50 ℃ 3 hours; with phenol - chloroform extraction DNA, 3mol / L sodium acetate , ethanol precipitated DNA, centrifugation after 70% of the
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