Dissertation > Excellent graduate degree dissertation topics show

Expression of Protein Kinase C Isforms in Chinese Hamster Ovary Cell and Xenopus Oocyte

Author: Dong（WangLe）Zuo
Tutor: ZhangHaiLin
School: Hebei Medical University
Course: Pharmacology
Keywords: Homology Protein kinase C Inward rectifier potassium channel RT-PCR Western blot
CLC: R33
Type: Master's thesis
Year: 2005
Downloads: 40
Quote: 0
Read: Download Dissertation

Abstract


Inward rectifier potassium channel (Inwardly Rectifying K Channel, Kir) is widely distributed in various tissues of a potassium channel , named for its voltage and current relationship with the inward rectifier characteristics . The main physiological function of the Kir channel is the maintenance of cell resting potential and mediated potassium ion transmembrane transport . Since 1996, Henry et al [1] found that since the Kir current inhibition of protein kinase C (PKC) expression in Xenopus laevis oocytes , PKC inhibition mechanisms of Kir channel current become one of the focus of the people . Most people think that PKC is a direct phosphorylation of the channel protein Kir channel function to adjust , but no exact biochemical evidence can not be conclusive . Our previous work , PMA ( myristoyl phorbol ethyl acetate ) to activate PKC inhibit Kir2.3 channel currents expressed in Xenopus oocytes can not inhibit the expression in CHO cells Kir2.3 channel current, but the mechanism of this difference is unknown . We suppose that one of the possible mechanisms for the two types of cells exist in different PKC isoforms . PKC is an important material in the cell signal transduction pathways , membrane phospholipids on the cell membrane PIP 2 IP3 and DAG generated after PLC hydrolysis , by raising intracellular Ca 2 the physiological functions of the cells and activation of PKC regulation. To date, at least 12 kinds of PKC subtypes have been isolated and purified . A variety of different biological characteristics of PKC isozymes of PKC family , whose members have different enzymatic properties of different tissues , also has a significant role in cell signaling . In recent years, the overall function of PKC

Related Dissertations

  1. Preliminary Study of Phosphorylation Effect on UGT1A3 Metabolism Activity Towards Drugs,R346
  2. Purification of Endogenous Proteases from (Litopenaeus Vannamei) Shrimp Head and Their Enzymology Properties,S985.21
  3. GIH cDNA Cloning and Mrna Expression in Litopenaeus Vannamei,S917.4
  4. Gene Prediction and Sequence Analysis of Insect OBP CSP and sid-1,Q78
  5. Isolation, Identification and Molecular Characteristics of Japane Encephalitis Virus in Henan Province,S852.65
  6. Studies on the Difference of PRRSV Infectious and PRRSV ADE,S858.28
  7. cDNA-cloning and Expression analysis of Phosphate Stress Induced Purple Acid Phosphatase Genes and transcription Factor Genes from Seeding Wheat,S512.1
  8. Study on the Transgenic Tobacco Expressing Protein Kinase Like Gene TA50-10 of Wheat and Analysis of Disease Resistance,S572
  9. Study on Key Technology and Its Physiological Basis of Virus Elimination and Rapid Propagation of Phalaenopsis,S682.31
  10. Molecular Mapping of Downy Mildew Resistant Gene and Expression of Rs-AFPs in Radish (Raphanus sativus L.),S631.1
  11. Roles of MMP-7 and Lysozyme in the Pathogenesis of Ulcerative Colitis Induced by DSS in Balb/c Mice,S858.91
  12. Proliferation and Differentiation Effects of Different Culture Methods and Cell Factors on Mouse Spermatogenic Cells in Vitro,R329
  13. The Effect and Mechanism of p38MAPK Inhibitor CBS3830 on Intimal Hyperplasia in Autogenous Vein Grafts of Diabetic Rats,R587.1
  14. The Detection and Significance of CK 19 mRNA and MUC1 mRNA in Peripheral Blood in Cervical Cancer Patients.,R737.33
  15. Kinase Activities Analysis and Localization of ZmCCaMK Induced by Abscisic Acid in Leaves of Maize Plants,S513
  16. Effect of HCO3-, K+ and HSO3- on rbcL and rbcS Gene Expression and Photosynthesis of Soybean Seedlings,S565.1
  17. The Impact of Histone Deacetylase Inhibitor Trichostatin a on Proliferation and Apoptosis of Porcine Granulosa Cells,S828
  18. Primary Study on CLC1 Gene Identification and Its Function in Glycine Max, Salt-born Glycine Soja and Their Hybrid,S565.1
  19. Cloning of Celluase Gene cel6B from Lentinula Endodes and Its Expression in Escherichia Coil,S646.12
  20. A Study of the Expressions of MT1FmRNA and VEGFmRNA in Acute Myelocytic Leukemia,R733.71
  21. The Study of Fluorescence Quantitative PCR for Detection of Streptococcus Pneumoniae in Sputum of Acute Exacerbations of Chronic Obstructive Pulmonary Disease,R563.9

CLC: > Medicine, health > Basic Medical > Human Physiology
© 2012 www.DissertationTopic.Net  Mobile