Dissertation > Excellent graduate degree dissertation topics show

Specific cyclooxygenase-2 inhibitors CELECOXIB gallbladder cancer cell lines in vitro induced apoptosis of GBC-SD and FK506 on Severe Acute Pancreatitis in Rats

Author: WangYuTong
Tutor: ZuoKeFeng
School: Fourth Military Medical University
Course: Surgery
Keywords: Cyclooxygenase-2 CELECOXIB Apoptosis
CLC: R735.8
Type: Master's thesis
Year: 2005
Downloads: 78
Quote: 0
Read: Download Dissertation

Abstract


Objective: This study was designed to study specific cyclooxygenase-2 inhibitors - CELECOXIB in vitro on human gallbladder cancer cell line GBC-SD growth inhibition , induce apoptosis . Observed at different concentrations through the inhibition of cell growth and apoptosis gallbladder function , lay the foundation for clinical application . Methods: a gallbladder cancer cell determined by counting growth curve obtained basic growth characteristics of the cell lines ; 2 gallbladder and cells were treated with different concentrations ( 0,40,80,160 and 320μmol / L) of the medium CELECOXIB training, 24 hours after the application of growth inhibition MTT assay , flow cytometry to observe apoptosis ; 3 gallbladder cancer cells containing 160μmol / L CELECOXIB the culture medium , respectively Hoechst33258 reagents and TUNEL staining method . Results: 1 CELECOXIB growth inhibition in gallbladder carcinoma cells , and showed volume - effect relationship. By flow cytometry , to observe significant apoptotic cells ; 2 Hoechst33258 reagents and by TUNEL staining , the presence of apoptotic cells found significant . Conclusion: CELECOXIB for gallbladder cancer cell has a certain tumor cells induced growth inhibition and apoptosis occurs , this effect was dose- dependent and time- dependent.

Related Dissertations

  1. Cloth and β- elemene combined administration of anti-tumor effect and mechanism of celecoxib,R96
  2. The Effects and Preliminary Study of PCV2 on Ca2+ Signal in Lymphocytes of Piglets,S858.28
  3. The Effects of (180F-FDG on the Apoptosis of Eca-109 Esophageal Cancer Cell Line,R735.1
  4. The Expression and Mechanism of NF-κ B and IL-6 after 90% Portal Vein Ligation in Rats,R657.3
  5. The Effect of Bidentata Saponins on Early Osteoarthritis in Rabbit and PI3K/AKT Pathway,R285.5
  6. Effects on Recombinant Human Interleukin-10 of IL-2, IFN-γ, GCs, T Cell Apoptosis in Transplantation of the Rabbit Skin,R392
  7. Effect of Chloride Channel Blocker on the Hippocampal Neuronal Apoptosis Induced by No in Culture,R363
  8. Expression of Caspase-4 and Caspase-3 Gene Related Apoptosis in Pathological Changes of Sternocleidomastoid of Congenital Muscular Torticollis and Four Years Clinical Followed-up for Opereated Patients,R726.8
  9. Expressions and Relationships of PI3K/AKT and Apoptosis Related Regulation Factors in Epithelial Ovarian Tumors,R737.31
  10. Pathological Changes of Cerebral Vasospasm and the Expression of Cell Apoptosis Related Factors JNK and AKT Following Subarachnoid Hemorrhage of Rats,R743.34
  11. Research on Apoptosis of Human Colon Carcinoma Cell HT-29 Induced by NDGA Combined Celecoxib,R735.35
  12. The Studies to Anti-tumor Effect and Its Mechanisms of Nsaids Combined with Sodium Butyrate on Human Colon Cancer Cell Line HCT-116 in Vitro,R735.35
  13. Edaravone promote functional recovery of sciatic nerve injury in early experimental study,R965
  14. Apoptosis Mechanisms Research in Interfering TIGAR Gene and Epirubicin Treatmnet in Hepatic Carcinoma Cells,R735.7
  15. The Studies of Celecoxib Affecting Its Expression Profiles of microRNA Mechanisms on Human Colon Cancer Cell Line HT-29 in Vitro,R735.35
  16. A Study on the Time-Dependent Expression of Caspase-8in Acute β-irradiated Skin Injury in Rats,R818
  17. Effects of Propofol on the Apoptosis and Activation of NF-κB in Polymorphonuclear Neutrophil in Acute Lung Injury Induced by LPS in Rats,R965
  18. The Expression of microRNA in the Ovary of Polycystic Ovary Syndrome Rat Model,R711.75
  19. Apoptosis of Medial Smooth Muscle Cells Elicits Recipient Bone Marrow Cells to Participate in Allograft Arteriosclerosis,R654.3
  20. Effects of Celecoxib on Proliferation,apoptosis and Nucleostemin Expression in Human Breast Cancer Cell Line SKBR3,R737.9
  21. The Influence of Cycloxygenase-2 Inhibitor celecoxib on the Growth of Hepatocellular Carcinoma Cell Line HepG2,R735.7

CLC: > Medicine, health > Oncology > Gastrointestinal Cancer > Gallbladder, bile duct cancer
© 2012 www.DissertationTopic.Net  Mobile