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Purpose of rhBMP-2 and bFGF as osteoinductive factor FG carrier engineered bone for spinal fusion rabbit transverse process to explore bFGF and rhBMP - 2 in the the rabbit transverse process spinal fusion whether there is synergy for spinal fusion to find a more effective bone graft substitute materials , to provide the basis for bFGF and rhBMP-2 in combination . , Animal groups 30 Japanese white rabbits were divided into three groups . Experimental group FG bFGF rhBMP - 2 , the control group 1 FG rhBMP- 2 , and the control group ( n = 2 ) for the FG . Vitro tissue engineering materials Construction of fibrinogen , thrombin , CaCl at 2 < / sub > and other materials by a certain method of preparation can be obtained FG . Add both bFGF and rhBMP-2 were used in the experiment group , the addition of rhBMP-2 is used to control group 1 . Third , the implant surgery revealed the left side of the fifth , sixth lumbar transverse process , burr to the rear of cortical bone rubbed off , exposing the cancellous bone . The prepared FG bFGF rhBMP-2, FG rhBMP-2, FG implanted in the left side of the corresponding groups of animals , the rear of the sixth lumbar transverse process . Spinal fusion six weeks after evaluation methods slaughtered animal was removed and lumbar spine specimens general observation , imaging observation , biomechanical testing , and histological observation of the data were statistically analyzed . Seven specimens left fifth and sixth lumbar transverse process fusion results of the test group and control group 1 4
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