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Extaction, Separation and Quality Analysis of Several Medicinal Substances in Tobacco

Author: WeiHua
Tutor: ZuoHeMingï¼›LiuZhengLiang
School: Second Military Medical University
Course: Pharmaceutical Analysis
Keywords: Tobacco Supercritical Fluid Extraction Column chromatography Solanesol Tobacco volatile oil Nicotine Tobacco polysaccharide Amino acids Qualitative and quantitative analysis
CLC: R284
Type: Master's thesis
Year: 2005
Downloads: 357
Quote: 1
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Abstract


Tobacco as a traditional Chinese medicine , only contained in the \This character is warm, spicy , a small drug. Swelling , detoxification , insecticide efficacy . Modern research has shown that the main chemical components of tobacco the alkaloids , rot amines, fatty alcohols , and other pharmaceutical substances . In this paper, samples collected from Shandong Province, China , and Cuba 's western province of tobacco (Nicotiana tabacum L) as the research object , the application of supercritical fluid extraction technology solanesol tobacco extract combined with column chromatography separation and purification to obtain the content 99% high purity solanesol . NP-HPLC and LC-MS analysis , the samples meet the quality requirements of the traditional Chinese medicine chemical markers ; using GC-MS, HPLC, UV and automatic amino acid analyzer system isolated tobacco extract volatile oil , nicotine , tobacco , polysaccharides , amino acids , etc. component Determination and analysis methods . Experimental results show that : solanesol content in tobacco samples and production in China and Cuba were 1.84% , 1.43% ; 9 and 14 components , each identified volatile oil , which accounted for 88.53% and 55.12% of the total volatile oil ; nicotine content 27.78% , 26.01% ; tobacco polysaccharide content of 17.21% , 15.01% ; total amino acid content of 4.46% , 12.41% . Above , including the obtaining of high purity solanesol , solanesol quantitative analysis method modified tobacco or waste system extraction methods of research are exploratory research carried out for the first time in this article , the results obtained are not yet see reported in the literature . This paper research on tobacco medicinal development and resources comprehensive utilization has guiding significance and practical value .

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CLC: > Medicine, health > Chinese Medicine > Of Pharmacy > Traditional Chinese medicine chemical
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