Dissertation > Excellent graduate degree dissertation topics show

The Study on the Isolation and Purification of Islets Cells from Human Pancreata and the Effects of Immunosupperssive Agents on Islets Cells

Author: WangZhen
Tutor: GaoJiangPing
School: PLA Postgraduate Medical School
Course: Department of Urology
Keywords: islet transplantation immunosuppression cell separation
CLC: R587.1
Type: Master's thesis
Year: 2005
Downloads: 94
Quote: 0
Read: Download Dissertation

Abstract


Objectives:1.To investigate how to improve the yield and quality of human islets in islolation and purification and evaluate the quality of islet cell .2.To investigate the effects of immunosuppressive drugs on the viability of pancreatic islets cells cultured in vitro ,and to explore their effects on the outcome of ilslet transplantation.Methods:1.Human’s islet cells were isolated using two times digestion by collagenase injected through pancreastic duct. Islet were purified by Ficoll-400 discontinous density gradient centrifugation and cultured. 2.The identification of islet cells and acinar cells were studied with dithizone (DTZ) and acridine orange (AO)-propidium iodide(PI). 3.Human islet cells were exposed to various concentrations of four immunosuppressive agents for 72 hr respectively. Glucose -stimulated insulin secretion during subsequent static incubation was measured using the ultrasensitive human insulin ELISA kit.Results:1.The mean yield of islet cells was 80 000 islets/pancreas.Islet purity was over 80%,islet viability was more than 95%.The insulin releasee test indicated the islet cells were sensitive to

Related Dissertations

  1. Study of Intracerebral Transplantation of Umbilical Cord Blood Mesenchymal Stem Cells in a 6-Hydropamine Rat Model of Pakinson’s Disease,R742.5
  2. Epidemiologic Studies on Duck Circovirus Infection in East China and Development of Monoclonal Antibody Against Duck Circovirus,S858.32
  3. A Model of Acute Interstitial Pneumonitis by Murine Cytomegalovirus Infection in Mice with Allogenic Skin Transplantation,R-332
  4. The Study of Immuno-tolerance Mechanism of the Third-party Bone Marrow-derived Mesenchymal Stem Cells on Allogenic Transplantation,R392
  5. The Effect of Exogenous Brain-derived Neurotrophic Factor on Transplantation Treatment of Bone Mesenchymal Stem Cells in Intracerebral Hemorrhage in Adult Rats,R743.34
  6. Study on Human Peripheral Blood Stem Cells Differentiate into Liver Cells,R575.1
  7. Experimental Investigation of Grafting Pathways of Bone Marrow Stromal Cells into Spinal Cord Injury in Rats.,R651.2
  8. Improvement of the Result of Autologous Fat Transplantation in Facial Recontouring Surgery by Using "Cocktail" Therapy,R622
  9. The Distribution of KIR Gene in Chinese Han Population and the Role in Unrelated Allogeneic Hematopoietic SCT,R392
  10. An Experimental Study on the Protective Effect of Edaravone on Liver Graft against in Rat OLT Model,R657.3
  11. The Research of Key Techniques on Computer-Aided Planning before Liver Transplantation,TP391.41
  12. The Study on the Radiosensitizing Effect and Mechanism of Artemisinin on Nude Mouse Transplanted with HeLa and Siha Cells of Human Cervical Cancer,R737.33
  13. Government ( police ) department Network Video Monitoring System Design and Implementation,TP277
  14. The Effect of Ang(1-7)/MAS Pathway on Pancreatic Islet Microcirculation and Function in Rat with Long-term High-fat Diet,R587.1
  15. Ang(1-7) Improves Pancreatic Islets Function Via Upregulation of eNOS Expression in Rats with High-fat Diet,R587.1
  16. Oridonin preconditioning on rat hepatic resection autologous transplantation ischemia -reperfusion injury in rats and its mechanism,R657.3
  17. Olfactory ensheathing cells promote experimental injury in rats cochlear spiral ganglion cell protection and repair,R764
  18. Human umbilical cord blood mononuclear cells , umbilical cord mesenchymal stem cell transplantation for treatment of neonatal rat model of cerebral palsy,R742.3
  19. Effect of Different Serum Levels of Tacrolimus to Transplant-Renal Doppler Resistance Indices,R699.2
  20. Renal hyperparathyroidism surgical treatment ( 9 cases and literature review ),R699
  21. Inhibiting the expression of islet grafts TF IBMIR animal models in vivo effects of acute studies,R587.1

CLC: > Medicine, health > Internal Medicine > Endocrine diseases and metabolic diseases > Islet disease > Diabetes
© 2012 www.DissertationTopic.Net  Mobile