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Study the purpose : Is it increase progressively load swim between sport and big mouse serum , sura skin , myocardium ROS , MDA content and SOD , GPX , influence , T-AOC of activation to probe into. Study content 1, : increase progressively through observation load swimming sport in, big mouse serum, cardiac muscle, skeleton muscle ROS, MDA content and SOD , GPX , influence , T-AOC active change, discuss free base produce and fight the relation of growth and decline between oxidize system. Study content 2, : observe increase progressively load swimming sport in big mouse serum, cardiac muscle and the change of skeleton muscle MDA, ROS , ROS/T-AOC, discuss preliminarily " free base threshold" exist possibility. Study content 3, : increase progressively fairly load sport process in SOD/ROS and GPX/ROS ratio with SOD and GPX the difference of active change, discuss as reflection organism fight the important parameter of oxidize ability, one more superior.Research approach: The big mouse increases progressively and shoulders a heavy task to swim, for 60 minutes. And then every sport group is it put according to sport way , sport of model to death at once after the one’s own arrangement to finish, fetch the blood , sura skin , cardiac muscle. After the big mouse buys, the new environment suits for one day. Divided into 7 groups at random: Contrast the group quietly (C, n =8), group (Ex1 , n , 10min of sport , =8), group (Ex2 , n , 20min of sport, =8), group (Ex3 , n, 30min of sport, =8), group (Ex4, n, 40min of sport, =8), group (Ex5 , n, 50min of sport, =8), group (Ex6, n, 60min of sport, =8),there is no significance difference in the weight among the groups. Acclimatization training of the big mouse : Is it is it swim one day to shoulder a heavy task to have, sport group old mouse is it exhaust to strength to swim. Shoulder a heavy task and swim for 7 days: The 1st, one day, each large mouse 2% weight is it is it exhaust to strength to swim to shoulder a heavy task; The third, one day, each large mouse 6% weight is it is it exhaust to strength to swim to shoulder a heavy task; The 5th, one day, each large mouse 10% weight is it is it exhaust to strength to swim to shoulder a heavy task. All sport old mouse swim time to be 60min about strength exhaust, strength exhaust timeto be very nearly the same.Result: Increase progressively 1, when swiming load serum, calf intestines muscle and the change serum MDA content of the MDA of cardiac muscle: Ex 1 compare group than peace go up somewhat, but do not have significance. Ex 2 ~ Ex 6 than for shine organize have very significance go up ( P < 0.01 ). calf intestines muscle MDA content: Ex 1 group comparatively Ex 3 compare go up somewhat, but not notable, Ex 4 group Ex 6 very notable rising( P < 0.01 ). the MDA content of cardiac muscle: Ex 4 with compare than very significance go up (P < 0.01); Ex 5 and Ex 6 with compare group than go up apparently( P < 0.05), more Ex 4 drop somewhat. Increase progressively 2, when swiming load serum, calf intestines muscle and the change of the ROS of cardiac muscle: serum ROS content change with MDA similar, however Ex 2 group change between Ex 5 less, after go up again, calf intestines muscle, the ROS content of cardiac muscle and sport organize and compare compare with change not obvious. Increase progressively 3, when swiming load serum, calf intestines muscle and the change of the SOD of cardiac muscle: serum SOD active: Ex 1 group comparatively Ex 6 compare low, however, Ex 1 very notable difference Ex 3 not notable; Ex 4 group comparatively Ex 6 have ( P < 0.01 ). calf intestines muscle SOD active: Ex 1 compare drop apparently( P < 0.05 ). Ex 2 group comparatively Ex 4 compare very drop apparently( P < 0.01), compare with Ex 1 without obvious change. Ex 5 go up somewhat than Ex 4, but with compare group than is still drop apparently( P < 0.05 ). Ex 6 than for shine organize have very significance drop ( P < 0.01), is smaller than Ex 5 just, the SOD of cardiac muscle active: Ex 1 very significance discrepancy Ex 3 with compare comparing with group without significance discrepancy, Ex 4 Ex 6 have ( P < 0.01 ). Increase progressively 4, when swiming load serum, calf intestines muscle and the change of the GPX of cardiac muscle: serum GPX active: Ex 1 group comparatively Ex 3 compare drop apparently( P < 0.05), Ex 4 , calf intestines muscle GPX with SOD active change Ex 6 very drop apparently( P < 0.01) the, sport difference of across block little, compare with active change and not obvious, the GPX of cardiac muscle active: Ex 1 and Ex 2 drop comparatively comparing with group, but do not become notable tendency. Ex 3 and 4 last litres of Ex, but have no notable tendency. Ex 5 and
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