Dissertation > Excellent graduate degree dissertation topics show

A Study on the Mutations in Exon 9 of LPL Gene

Author: ZhaoYu
Tutor: JieYongHong
School: Tianjin Medical University
Course: Biochemistry and Molecular Biology
Keywords: lipoprotein lipase (LPL) gene mutation polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) hypertriglyceridemia
CLC: Q754
Type: Master's thesis
Year: 2005
Downloads: 45
Quote: 0
Read: Download Dissertation


Lipoprotein lipase (LPL) plays an important role in lipoprotein metabolism by hydrolyzing triglyceride-rich lipoproteins such as chylomicrons and very low density lipoprotein. The major deficiency of LPL can cause hypertriglyceridemia, which is often the result of gene mutations. LPLSer447Ter found in 1990 is a consequence of a C→G transversion at nucleotide 1595 in exon 9 of LPL gene, converting the serine 447 codon (TCA) to a premature termination codon (TGA). Many researchers discovered that Ser447Ter group was associated with lower TG levels and higher HDLc levels than the controls, and to compare with the coronary group, frequency of this mutation is higher in the non- coronary group. Ser447Ter variant has been reported to have beneficial effect on TG and HDL-c in patients with CHD or normal subjects. But there are contrary opinions, the influence of this mutation upon the blood lipids exists the controversy. To investigate distribution of gene mutations in LPL exon 9 in Chinese population and to study the possible effects of the mutation on plasma lipids, variants were screened by PCR-SSCP and DNA sequencing. Ser447→stop polymorphism was identified by PCR-RFLP . the plasma lipids in stop447 carrier group and stop447 noncarrier group were compared and analysis.The frequencies of stop447 allele is 9.4% in the 420 subjects with normal TG

Related Dissertations

  1. The Study of Mutation in the CRX Gene Associated with Late-onset Genetic Retinitis Pigmentosa (RP),R774.1
  2. Influences of Temperature on SCF/c-kit Prolifertation Pathway and Cell Cycle of Rat Spermatogonial Cells Cultured in Vitro,R698
  3. The Study of Clinical Chracteristics and Pathogenesis of Thrombotic Thrombocytopenic Purpura,R554.6
  4. Qingdao region of hepatitis B virus the BCP gene mutation and hepatitis B virus associated with primary liver cancer,R512.62
  5. Aanalysis of Genotype and ORF3 Gene Mutation of Hepatitis E Virus in Wuhan,R512.6
  6. Study on the Association of the Precore/Core Region Variations of Hepatitis B Virus and Hepatocellular Carcinoma in Qidong High-Risk Area,R735.7
  7. The Analysis and Significance of JAK2 and TET2 Gene Mutation in Myeloproliferative Neoplasm,R733.3
  8. An idiopathic Brugada syndrome ECG levy molecular genetic analysis of pedigree,R541.7
  9. Clinical Study on Curing Hyper-triglyceride and Obesity by Catgut Implantation at Acupoint,R246.1
  10. Expression of Bovine Scrapie Prion Protein Gene PrP102-242 and PrP1-264 in COS-7 Cells,S852.659.7
  11. Mutational Analysis of SLC26A5 IVS2-2A>G in Chinese Han Patients with Non-syndromic Hearing Loss,R764
  12. Effect of Once High-dose Coenzyme A on Postprandial Hypertriglyceridemia after a High-fat Meal,R589.2
  13. Molecular Genetic Analysis of a Chinese Family with Tuberous Sclerosis Complex,R593.2
  14. Study of Sulfurmetabolism Function for luxS Gene of the Streptococcus Mutans,R781.1
  15. Bioinformatic Study on the Structure of Human Lactate Dehydrogenase C4 and Construction of Recombinant Vectors for the Expression of the L178X Mutant,Q78
  16. Neisseria gonorrhoeae IR base deletion in its multi-drug resistant,R379
  17. The Detection of Follicular Stimulating Hormone Receptor Gene C1555A Mutation and Luteinizing Hormone Receptor Gene C1660T Mutation in Premature Ovarian Failure,R711.75
  18. Patients with congenital ventricular septal defect closure GATA4 gene mutations and changes in cardiac structure and function after surgery follow-up study,R541.1
  19. Study on HIV-1 Drug-resistant Mutation Status in Fujian Province and Subtype Analysis of HIV-1 Strains Circulated in HIV-1 Infected MSM,R512.91
  20. Study on the Correlation between mRNA Expression and Mutation of SORL1 Genes and Alzheimer Disease,R749.16
  21. Establish of the Standard Sequence of Full-length Hepatitis B Virus Genome and Associtions Between Mutations and Hepatitis B Virus Related Disease,R512.62

CLC: > Biological Sciences > Molecular Biology > Molecular Genetics > Gene structure and mutations of the molecular principles of
© 2012 www.DissertationTopic.Net  Mobile