Dissertation > Excellent graduate degree dissertation topics show

Photometric Method Study on the Reaction Mechanism of Small Organic Molecules with Proteins

Author: SunYanMei
Tutor: LiuBaoSheng
School: Hebei University
Course: Analytical Chemistry
Keywords: photometric method Serum albumin reaction mechanism
CLC: O629.73
Type: Master's thesis
Year: 2005
Downloads: 243
Quote: 1
Read: Download Dissertation

Abstract


This dissertation studies the reaction mechanism of some organic molecules, Alizarin red, acidic phenolsulfonphthaleins, Aesculin and Aesculetin with protein. Those reaction accord with Langmuir equation, quenched the fluorescence produced,at the same time content of Aesculin in cortex was directly mensurated , which did not separate by TLC. The detail as following Chapter one: The results and methods for study of reaction mechanism between small organic molecules with protein were reviewed with 55 references. Chapter two: The microphase absorption—spectral correction （MPASC） technique is described and applied to the study of the interaction of Alizarin red （ARS） with two proteins : bovine serum albumin （BSA） and human serum albumin （HSA） at pH 4.3 Britton―Robinson buffer solution . The results show that the absorption ratios of ARS to proteins are NARS-BSA = 9∶1,NARS-HAS = 7∶1,the absorption constants are KARS-BSA = 3.346×104,KARS-HAS = 4.483×104 respectively and the real molar absorptivities are measured to be εBSA-ARS（537 nm） = 2.517×104 L·mol-1·cm-1,εHSA-ARS（519 nm） = 2.051 ×104 L·mol-1 ·cm-1 . The detection limits are 19 mg·L-1 for BSA , and 23 mg·L-1 for HSA.The aggregation of ARS in protein has been applied to the analysis of samples with satisfactory results. Chapter three: The reactions between HSA and acidic phenolsulfonphthaleins including phenol red, cresol red, chlorophenl red, bromocresol purple and m-cresol purple have been investigated by the means of fluorescence. The experiments showed that all of the selected acidic phenolsulfonphthaleins strongly quenched the fluorescence produced by HSA, and this fluorescence quenching could be interpreted in terms of statistic quenching. From the calculation results of binding constants K for these dyes at various temperatures, it could be found that the increase in the functional groups numbers of dyes molecules resulted in an increase in K, while the increase in reaction temperature led to a decrease in K. The interactions between dyes and HSA were attributed to static-electricity gravitation which was confirmed by the calculation results of enthalpy and entropy for these reactions. According to

Related Dissertations

  1. Spectral and Electrochemical Research of Epirubicin Hydrochloride with Its Analytical Application,TQ465
  2. Bovine serum albumin and protamine from NOCC / alginate hydrogels release : the isoelectric point of two different biological molecules controlled trial,R943
  3. Containing pyridyl group metal complexes Synthesis and Structure,O627
  4. N-heptane HCCI combustion process temperature thermodynamics and kinetics theory,O643.21
  5. Theoretical Study on Three Miportant Reactions Catalyzed by Nano-Gold Catalysts,O643.32
  6. Research on the Synthesis of Methyl Pyruvate,TQ225.241
  7. Early Cytogenetic Damage of Plywood Workers Occupationally Exposed to Formaldehyde,R131
  8. Kinetics of Catalysis Hydrolytic Depolym Erization of Poly (ethylene Terephthalate) Under Microwave Irradiation,O631.3
  9. The Mechanism of the Degradation of DNS and Its Kinetics,O643.12
  10. Mechanism Study on Mercury Homogeneous and Heterogeneous Reaction,O643.12
  11. Oxidation of arsenic-containing copper sulfide concentrate roasting,TF811
  12. Study on Forming Mechanism of the Fe-Al2O3-FeAl2O4 Composite Coating by Reactive Plasma Spraying,TG174.442
  13. Process and Properties Research of the SiO2f/SiO2-BN Composites by Urea Route,TB332
  14. The Applications of L-cysteine Capped CdTe Quantum Dots as Fluorescent Probes,R341
  15. Chitosan/Bovine Serum Albumin Co-micropatterns on Titanium Surfaces and Their Biomedical Performance,TB383.1
  16. Synthesis and Study of Interaction between Luminescent Acceptor Based on Stilbene Derivatives and Protein,O621.3
  17. Studies of the Interaction of Several Antimicrobial Drugs with Bovine Serum Albumin Using Spectroscopy,R96
  18. The Theoretical Investigation of Oxidative Kinetic Resolution of Secondary Alcohols Catalyzed by (-)-sparteine-Pt Complexes,O621.25
  19. Calcium-based chemical looping combustion process of oxygen carrier,O643.21
  20. Inteaction of Resveratrol、Psoralen and Ursolicacid with Protein,R285
  21. Ni-YSZ/YSZ/LSM solid oxide fuel cell reaction mechanism of Monte Carlo simulation,TM911.4

CLC: > Mathematical sciences and chemical > Chemistry > Organic Chemistry > Natural compounds > α-amino acids,peptides, proteins, nucleic acids > Protein
© 2012 www.DissertationTopic.Net  Mobile