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Tobacco alkaloids, sterols and non- volatile acids by gas chromatography / mass spectrometry

Author: XuYanJuan
Tutor: TangWanYing；XuGuoWang
School: Nanjing University of Technology and Engineering
Course: Environmental Engineering
Keywords: Tobacco Analysis Alkaloids Sterol Non- volatile acid GC / MS
CLC: TS41
Type: Master's thesis
Year: 2005
Downloads: 362
Quote: 0
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Abstract


Tobacco is a leaf crops, the quality of tobacco and its products are mainly composed of its intrinsic chemical composition content of the decision. Changes in chemical composition of tobacco is extremely complex , tobacco types, differences in chemical composition . Based on the pre-treatment method and the column system selection comparison and study , by solvent extraction and gas chromatography of the tobacco alkaloid and sterols , derivatization gas chromatography analysis of the non- volatile acid tobacco , with retention time and relative retention times and by GC / MS , internal standard . Alkaloids measurement results relative standard deviation of 2.2 to 15.0 % and the method recovery was 89.4 ~ 98.8% . Sterol measurement results relative standard deviation of 2.6 to 8.8% , recoveries of 92.7 ~ 102.1% . Non- volatile acid determination results relative standard deviation of 0.6 to 19.8% recoveries of 86.7 ~ 103.6% . In the study , based on the Changde Cigarette Factory provides 52 samples of tobacco alkaloids, sterols were relatively non- volatile acid content and comparison of different tobacco leaves found mainly alkaloids, sterols, relatively non- volatile acid content is quite different, the total alkaloids of 0.92 to 3.03 % of total sterol 0.02 to 0.06% of total non- volatile acid content of 2.31 ??~ 4.55% . The conclusions of this intelligent system development cigarette formulation provides a theoretical basis for achieving the tar harm reduction , and promote modernization of business and industry has a positive meaning .

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CLC: > Industrial Technology > Light industry,handicrafts > Tobacco Industry > Basic science
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