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Studies on Creation Technique of Germplasm Resources in Roses

Author: HanJin
Tutor: FengZhen
School: Shandong Agricultural University
Course: Forest cultivation
Keywords: Chinese rose Cbf1 Germplasm Resources Pollen tube pathway
CLC: S685.12
Type: Master's thesis
Year: 2005
Downloads: 479
Quote: 4
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Abstract


The two aspects of this study from the conventional techniques and genetic engineering to explore innovative technologies rose germplasm resources, try to create new variant of the rose, rose breeding for the future provision of new the germplasm or further developed into new varieties. The trial to explore the Rose hybridization hybrid sterility problem, and a number of measures to improve the hybrid seed rate; pollen tube pathway Rose genetic engineering, gene into the cbf1 to Rose which breed antifreeze better germplasm; finally rose germplasm in vitro culture preservation research, and other methods of genetic transformation of rose and germplasm conservation to provide material and technical support. Sucrose concentration of 5-8% medium suitable for the most rose varieties pollen germination, but the different varieties of pollen germination rate is a significant difference in the in vitro culture process, of some pollen germination rate is very low, it may be caused by hybridization One of the reasons Abortion. The data results show that the temperature is the main factor affecting the germination of pollen, 25 ℃ is the optimum temperature for pollen germination. Repeated pollination, hormone treatments help overcome hybrid affinity. In 2004, 35 hybrid combinations pollination 376, get 18 fruit, 110 seed. 2005 7 hybrid seedlings morphological observation and DNA technology combined to identify hybrid, initially identified six sample strains is a true hybrid. In 2004, through the pollen tube pathway smear 233 48 access to seed, seedling emergence of 21, 15 seedlings can not survive freezing detect DNA molecules 6 resistant seedlings, initially identified 2 samples strain containing cbf1 gene validation Pollen Tube Pathway target gene in rose breeding. We introduce the rose varieties 'Red Hat', 'Cardinal', 'acrobatics' tissue culture experiments, proved on MS medium containing hormones, three varieties of stem segments can germinate normally rooting. Proved by orthogonal experimental design: MS 6-BA (1.0 mg / L) of NAA (0.10 to 0.30mg / L) to meet the needs of the Rose tissue culture, to obtain a higher rate of bud induction; MS NAA (0.5mg / L ) 6-BA (1.0mg / L) can get the highest rate of callus induction and callus fresh weight, 1/2MS IBA (0.5mg / L) is the best medium to promote root growth test research to provide technical support to the Rose germplasm preservation.

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CLC: > Agricultural Sciences > Gardening > Ornamental Horticulture ( flowers and ornamental trees) > Flower trees in class > Rose,Rose,Rose
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