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Objective: study of recombinant human endostatin , mice pharmacokinetics and in vivo tissue distribution and excretion characteristics provide the basis and reference for clinical medication safety and rational use of drugs . Methods: four groups of rats , each group of seven . Divided into high , in the low-dose group and intravenous group were measured after administration of subcutaneous administration of high , medium and low radioactive dose group activity and intravenous administration rats blood radioactivity ; another , and then take four rats respectively, subcutaneous and intravenous administration, the different points of time was measured by SDS-PAGE electrophoresis 125 I - Endostatin radioactivity accounted for the total radioactivity in the sample ratio, further obtaining its pharmacokinetic The pharmacokinetic parameters . 5 groups of mice , each group of eight . After subcutaneous administration , were sacrificed after 5 time points , remove the heart, liver, lung, spleen , kidney , large intestine , small intestine , brain , muscle , blood , and its radioactivity was measured after weighing . Eight rats , after subcutaneous administration of bile from the common bile duct intubation collection . Measured radioactivity cumulative excretion of time to make plans in a different time . Take mice 10 after subcutaneous administration , urine and faeces were collected and radioactivity was measured at 6 time points , respectively . Results: rat Endostatin kinetic parameters , such as Table 9 - as shown in Table 15 . As can be seen from the table , subcutaneous administration of t max and C Max : 0.55H, 10.2ug/mg ( high dose ) ; 0.65h 4.85ug / mg ( medium dose ) , 0.81h , 3.22ug/mg ( low dose ) , the C max and dose-related . And t 1/2 β for of 6.46h ( high dose ) , 8.61h ( medium dose ) and 10.83h ( low dose) . t 1/2 β intravenous administration of 6.5 times . And this corresponds to the MRT were 11.0h 17.9h 15.7h, low-dose intravenous administration of 6.8 times . Tissue distribution in mice : Mice administered subcutaneously ,
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