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Background of severe hepatitis or acute liver failure in a higher incidence of the pathological features large blocks or sub-chunk of liver necrosis, and the residual hepatocyte proliferation was inhibited by a variety of factors, therefore dependent on the ability of the diseased liver caused by liver regeneration difficult. Orthotopic liver transplantation (OLT) is an effective method of treatment of end-stage liver disease to date, but the donor shortage, expensive and require lifelong use of immunosuppressive agents, and many other factors limit its clinical application. Hepatocyte transplantation as adjuvant therapy is an important supplement of the liver transplant, but the source of liver cells, the number of liver cells after transplantation in vivo proliferation and function has not been completely resolved, hinder its development, in theory, with normal liver cells as a source of cell transplantation is the most ideal, but there are also the problem of the shortage of donor and poor proliferative capacity; xenogenic hepatocyte wide variety of sources, but the existence of the problem of immune rejection, virus infections, especially retroviral infection; liver tumor cells Proliferation ability, but there is a risk of tumor implantation, and poor liver cell function; immortalized cell lines the most widely SV40 LT gene transfected immortalized liver cells, to solve the proliferation problem, but security was questioned. Human fetal liver cells secrete a growth factor to stimulate the growth and proliferation of cells in vitro tissue differentiation and proliferation ability, and weak immunogenicity, as a better source of cells; clinical data confirmed fetal liver cell transplantation in the treatment of liver failure, congenital The enzyme deficiency and other diseases have a certain value, but there are also the problem of lack of donor and transplanted cell proliferation difficulties. While liver stem cells can solve these problems, is because it has a strong proliferative capacity and bi-directional differentiation potential transplant only mature liver cells the number of 1/3 to 1/5, and fetal liver stem cell volume more easily from the portal vein injection easy to integrate into the hepatic plate after transplantation, there are easy to mass separation of culture and genetic manipulation. Studies have shown that a limited number of stem cells in the growth of quiescent adult liver, rich liver stem cells in the fetal liver, unknown pathogens and foreign genes into the human body can be avoided if selected human fetal liver stem cells as a source of cell transplantation, therefore, in vitro successful separation and purification of human fetal liver stem cells, and amplification of its culture undoubtedly has a very important significance. The experiment of human fetal liver stem cells were isolated and cultured amplification preliminary exploration, aimed at finding its into
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