Dissertation > Excellent graduate degree dissertation topics show

BMP2 and VEGF165 Genes Transfected Rat Bone Marrow Mesechymal Stem Cells Simultaneity Expression

Author: ShiLei
Tutor: TianXiaoBin
School: Zunyi Medical College,
Course: Surgery
Keywords: bone morphogenetic protein 2 vascular endothelial growth factor rat bone marrow mesenchymal stem cells
CLC: R329
Type: Master's thesis
Year: 2011
Downloads: 1
Quote: 0
Read: Download Dissertation

Abstract


Objective:To establish the method of isolation and cultivation of rat bone marrow mesenchymal stem cells(BMSCs) in vitro.To evaluate the simultaneity expression efficacy of rat bone marrow stromal cells(BMSCs) transfected with bone morphogenetic protein 2(BMP2) and vascular endothelial growth factor(VEGF) genes.Methods:BMSCs from rats were isolated and cultured in vitro by using the whole bone marrow adherence method.The BMSCs were transfected with BMP2 and VEGF165 by liposome-mediated method.The co-espression of BMP2 and VEGF165 genes were determined by Enzyme-linked immuno sorbent assay (Elise) and immunohistochemical analysis.Results:The cells transfected with BMP2 and VEGF165 indeed could produce the proteins of BMP2 and VEGF165.Conclusion:the BMSCs transfected with BMP2 and VEGF165 genes will be expression simultaneously of rat bone marrow mesenchymal stem cells.

Related Dissertations

  1. The Expression of MMP-2、 VEGF and Ki-67 and Human Glioma Grade,R739.41
  2. The Research on Treatment of Lentivirus-mediated hVEGF-165 Gene Transferring Mesenchymal Stem Cells on Rat Hind Limb Ischemia,R329
  3. Application Adipose-devired Stem Cells and Silk Fibroin and Chitosan Scaffold Materials Building Common Cells-stents Complex Experiments,R329
  4. Expression of TNF-α, MMP-9 and VEGF in BALF of Rats with Emphysema and Their Mutual Relationship,R563.3
  5. Influence and Significance of MMP-2 and VEGF of Hemangioma Endothelial Cells in Vitro Culture by Propranolol,R739.5
  6. The Investigation of Relationship between Therapeutic Effect and Prognosis and Changes of Peripheral Blood Vascular Endothelial Growth Factor and Metallothionein in Patients with Advanced Lung Cancer,R734.2
  7. Study on the Correlation btween Serum, Aqueous Humor VEGF, IL-6 Levels and the Neovascular Glaucoma,R775
  8. The Analysis of the Growth Factor Related with the Secondary Glaucoma in the Anterior Champer of Silicone Oil-filled Eyes,R779.6
  9. Vascular endothelial growth factor on endothelial cells concentration of magnesium ions,R965
  10. Non-small cell lung cancer with BMP-2 expression and significance of CD105,R734.2
  11. Experimental Studies on Therapy of Lung Cancer by VEGF-C siRNA Mediated by Liposomes,R734.2
  12. Construction and Identification of PIRES-BMP2-TGFβ3 Bicistronic Eukayotic Expression Vector,R87
  13. Meningiomas and MMP-9, VEGF expression and tumor angiogenesis and recurrence,R739.45
  14. Vascular Endothelial Growth Factor Gene Transfection Bone Marrow Stromal Cells Establish Scaffold to Treat the Ischemic Necrosis of Lunate Bone of Dogs,S858.292
  15. The Effect of Wnt3a Factor and Bone Morphogenetic Protein-2 Co-regulating Bone Marrow Mesenchymal Stem Cell Proliferation and Osteogenic Differentiation,R329
  16. Experimental Study of the Relationship between Pulmonary Metastasis in Osteosarcomas and Vascular Endothelial Growth Factor Expression and Microvessel Density,R738.1
  17. The Effect of IL-17 on VEGF Expression in Human Nsclc Cell in Vitro,R734.2
  18. Propranolol for Haemangiomas-insight into VEGF/ERK Signaling Transduction in HUVEC-12 Cells,R965
  19. The Mechanism of Triamcinolone Acetonide in Inhibiting Retinal Neovascularizations in Oxygen-Induced Retinopathy Models,R774.1
  20. Effect of Candesartan on Expression of VEGF and MCP-1 in Rat Retina with Diabetetes Mellitus,R774.1

CLC: > Medicine, health > Basic Medical > Human morphology > Human histology
© 2012 www.DissertationTopic.Net  Mobile