Dissertation > Excellent graduate degree dissertation topics show

Changes and Clinical Significance of Krüppel-like Factor2, Endothelial Nitric Oxide Synthase and Vascular Endothelial Growth Factor in Children with Kawasaki Disease

Author: LiuZhuRong
Tutor: PiGuangHuan
School: North Sichuan Medical College
Course: Pediatrics
Keywords: Kawasaki disease coronary artery lesion non-coronaryartery lesion Krüppel-like factor2 endothelial nitric oxide synthase vascular endothelial growth factorKawasaki disease Krüppel-likefactor2 vascular endothelial growth factor intravenous immunoglobulin
CLC: R725.4
Type: Master's thesis
Year: 2014
Downloads: 1
Quote: 0
Read: Download Dissertation

Abstract


Objective:To explore the role of Krüppel-like factor2(KLF2), endothelial nitricoxide synthase (eNOS) and vascular endothelial growth factor (VEGF) inKawasaki disease and coronary artery lesion.Methods:48Kawasaki disease patients (13patients with coronary artery lesions,35patients without coronary artery lesions)、24febrile controls and22healthycontrols were enrolled into the study. The expression of KLF2mRNA of theperipheral blood mononuclear cells(PBMCs) were measured using real-timepolymerase chain reaction (RT-PCR),and plasma KLF2, eNOS and VEGFlevel were measured using enzyme-linked immunosorbent assay (ELISA).Results:1. The expression of KLF2mRNA of the PBMCs in patients withcoronary artery lesions is lower than those in patients without coronary arterylesions(P<0.05) and is significantly lower than the febrile controls andnormal controls (P<0.01). The expression of KLF2mRNA of the PBMCs inpatients without coronary artery lesions is significantly lower than the febrilecontrols and normal controls (P<0.01). And the expression of KLF2mRNAof the PBMCs in patients with coronary artery lesions has a negativelycorrelation with Erythrocyte sedimentation rate(r=-0.609, p=0.027). 2. The plasma concentrations of KLF2in patients with coronary arterylesions is lower than those in patients without coronary artery lesions (P<0.05) and is significantly lower than the febrile controls and normalcontrols(P<0.01).The plasma concentrations of KLF2in patients withoutcoronary artery lesions is significantly lower than the febrile controls andnormal controls(P<0.01). And the plasma concentrations of KLF2inCALs group has a positive correlation with serum albumin(r=0.655, p=0.021).3.The expression of KLF2mRNA of the PBMCs has a positivecorrelation with the plasma concentrations of KLF2in KD(r=0.345, p=0.029).4. The plasma concentrations of eNOS in patients with coronary arterylesions is lower than those in patients without coronary artery lesions (P<0.05), and is significantly lower than the febrile controls and normal controls(P<0.01).The plasma concentrations of eNOS in patients without coronaryartery lesions is significantly lower than the febrile controls and normalcontrols(P<0.01).5. The plasma concentrations of VEGF in patients with coronary arterylesions is higher than those in patients without coronary artery lesions (P<0.05), and is significantly higher than the febrile controls and normalcontrols(P<0.01).The plasma concentrations of VEGF in patients withoutcoronary artery lesions is significantly higher than the febrile controls andnormal controls(P<0.01).And the plasma concentrations of VEGF in thefebrile controls is higher than normal controls(P<0.05).6. There is no correlation relationship among the expression of KLF2 mRNA of the PBMCs or the plasma concentrations of KLF2, eNOS andVEGF.Conclusion:Changes of KLF2, eNOS and VEGF may be involved in thepathogenesis of Kawasaki disease and participate in the development ofcoronary artery lesion in KD. Objective:To explore the mechanism of intravenous immunoglobulin (IVIG) in thetreatment of Kawasaki Disease and the effect of IVIG on KLF2, eNOS andVEGF in Kawasaki Disease.Methods:A total of48children with KD who received IVIG treatment wereselected in the study,22age-matched healthy children were selected as thecontrol group.The expression of KLF2mRNA of the PBMCs were measuredusing RT-PCR,and plasma KLF2, eNOS and VEGF level were measuredusing ELISA.Results:1. There is on significant difference between the expression of KLF2mRNA of PBMCs before and after IVIG treatmen(tP>0.05).Compare to theconvalescence group, The expression of KLF2mRNA of the PBMCs inpost-treatment is lower(P<0.01)and the control group is higher (P<0.05).2. There is on significant difference between the plasma concentrationsof KLF2before and after IVIG treatment (P>0.05). The plasmaconcentrations of KLF2in convalescence is higher than post-treatment(P<0.05),and is lower than control group(P<0.05).3. The plasma concentrations of eNOS post-treatment is higher thanpre-treatment. The plasma concentrations of eNOS in convalescence is higher than post-treatment(P<0.05),and there is on significant difference of theplasma concentrations of eNOS in convalescence and control group(P>0.05).4. The plasma concentrations of VEGF post-treatment is lower thanpre-treatment (P<0.05). The plasma concentrations of VEGF inconvalescence is lower than post-treatment(P<0.05),and there is onsignificant difference of the plasma concentrations of VEGF in convalescenceand control group(P>0.05).Conclusion:IVIG treatment affects both the acute and convalescent of eNOS、VEGF.For KLF2, IVIG treatment plays a significant role only in convalescent stage,Increase the expression of KLF2may play an important role in the treatmentof Kawasaki disease.

Related Dissertations

  1. The Expression of MMP-2、 VEGF and Ki-67 and Human Glioma Grade,R739.41
  2. The Research on Treatment of Lentivirus-mediated hVEGF-165 Gene Transferring Mesenchymal Stem Cells on Rat Hind Limb Ischemia,R329
  3. BMP2 and VEGF165 Genes Transfected Rat Bone Marrow Mesechymal Stem Cells Simultaneity Expression,R329
  4. Expression of TNF-α, MMP-9 and VEGF in BALF of Rats with Emphysema and Their Mutual Relationship,R563.3
  5. Influence and Significance of MMP-2 and VEGF of Hemangioma Endothelial Cells in Vitro Culture by Propranolol,R739.5
  6. The Investigation of Relationship between Therapeutic Effect and Prognosis and Changes of Peripheral Blood Vascular Endothelial Growth Factor and Metallothionein in Patients with Advanced Lung Cancer,R734.2
  7. Study on the Correlation btween Serum, Aqueous Humor VEGF, IL-6 Levels and the Neovascular Glaucoma,R775
  8. The Analysis of the Growth Factor Related with the Secondary Glaucoma in the Anterior Champer of Silicone Oil-filled Eyes,R779.6
  9. Vascular endothelial growth factor on endothelial cells concentration of magnesium ions,R965
  10. Epidemiologic Pictures of Kawasaki Disease in the Children’s Hospital Affiliated to Soochow University from 2006 through 2010,R725.4
  11. A Preliminary Exploration of Laboratory Diagnosis and IVF Improvement Protocol on Endometriosis,R711.71
  12. The Expression and Clinical Significance of VEGF and GST-π in Renal Cell Carcinoma,R737.11
  13. The Study of Expression of Myeloid-related Protein-8 and -14, CD11b and MMP-9 in Kawasaki Disease,R725.4
  14. Experimental Studies on Therapy of Lung Cancer by VEGF-C siRNA Mediated by Liposomes,R734.2
  15. Nitric Oxide and Its Synthetase on Alkali Burn Induced Corneal Neovascularization,R779.1
  16. Changes of Circulating Endothelial Microparticles in Patients with Kawasaki Disease,R725.4
  17. The Effects of the VEGF Different Genotype Carriers on the Hypoxia Training to Acute Mountain Sickness,G804.2
  18. Dynamic Observation on the Plasma Level of Vascular Endothelial Growth Factor and Matrix Metalloproteinases in Advanced Hepatocellular Carcinoma Patients after Transarterial Chemoembolization Combined with Infusion of Endostar,R735.7
  19. Effects of Acute Phase Serum of the Children with Kawasaki Disease on the Human Umbilical Vein Endothelial Cells Function,R725.4
  20. APS patients with type 2 diabetes and the concentration of NO in endothelial progenitor cells PI3K/AKT/eNOS signaling pathway,R285.5
  21. Meningiomas and MMP-9, VEGF expression and tumor angiogenesis and recurrence,R739.45

CLC: > Medicine, health > Pediatrics > Children within the science > Pediatric cardiac and vascular disease
© 2012 www.DissertationTopic.Net  Mobile