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Objective: To study MG132 of HepG2 cells UPP and MDR gene expression , explore the and MG132 induced HepG2 cells apoptosis mechanism . Methods: Using flow cytometry , Hoechst33258 staining , acridine acridine orange (AO) staining to detect apoptosis in HepG2 cells ; using RT-PCR was used to detect UPP related genes E1 , E2, E3 , 26S And proteasome , p53, activation of Caspase3 and MDR mRNA expression ; immunocytochemistry observed p53 , activation of Caspase3 P-gp protein expression changes ; detection of P-gp protein expression by Western-Blot ; concentration using high performance liquid chromatography assay intracellular table doxorubicin (EPI) . Results: flow cytometry , the proteasome inhibitor MG132 (2μmol / L, 5μmol / L) for 24h HepG2 cell apoptosis rate increased . Cells staining nuclear enrichment , fragmentation characteristics of apoptosis . MG132 treated HepG2 cells UPP related genes E1 , E2, E3, 26S And proteasome mRNA expression decreased of p53 , Caspase3 mRNA levels and protein levels . 1μmol / L the MG132 with 1μg/ml epirubicin combination can enhance the killing effect on HepG2 cells , the two can be combined to achieve the the single use 2μg/ml epirubicin induced apoptotic effect ; MG132 could down epirubicin the HepG2 cells MDRmRNA transcriptional level after processing and P-gp protein expression . Epirubicin combined with a small dose of epirubicin and MG132 treated cells compared with epirubicin group content increased.
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