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Background and Purpose: protease activated receptor (Protease Activated Receptors, PARs) belong to the G protein-coupled receptor family members , distributed in a variety of cells . In human and mouse were found in four kinds of PARs, of PAR- 1, PAR -2 , PAR -3 and PAR -4 . PARs can be activated multiple protease involved in the body's various physiological and pathophysiological processes . At present , more and more studies show PARs plays an important role in inflammation and allergy . The basophil is an important immune cells . The cell surface expression of the high affinity IgE receptor (FcεRI ) , and under the corresponding allergens cause receptor crosslinking , resulting in cell degranulation and thus the release of a variety of media , including histamine , IL -4 , IL -13 , white three the the alkenyl , prostaglandin E 2 < / sub> , etc. , through the direct effect of these media or activate other cells and thus participate in the process of inflammation , and allergy such . Although the role of PARs in inflammation and allergy has become a hot topic , but so far , basophils PARs research has not yet been reported . Four kinds of PARs expression on basophils , PARs activation of basophils with or without regulation ? These questions remain to be answered . The PARs human cell histamine release from basophils research PARs function will help people to understand , and is of great significance to further elucidate the role of basophils in allergic reactions occur . Materials and methods: using TRIZOL reagent was extracted from human basophil cell line KU812 total RNA, and then RT-PCR performed
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