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Objective: To study bone marrow mesenchymal stem cells in vitro and in vivo immunomodulatory effect and its mechanism . Method: cultured mesenchymal stem cells isolated from embryonic Wistar rat femur , sorted by cloning cells were highly homogeneous flow cytometry surface markers to identify its purity . The isolation and culture of mesenchymal stem cells were seeded in 96-well plates , and wait until the 80 % to 90% of the area covered hole to 30GY dose irradiation with a linear accelerator , and then isolated peripheral blood lymphocytes co-cultured 3d, cultured alone lymphocytes as control joined ConA stimulation 68h , respectively, using the MTT assay and ~ 3H-TdR assay of MSCs on peripheral blood lymphocyte proliferative responses . Human peripheral blood lymphocytes using RT-PCR and Real-time PCR detection by MSCs treatment of TGF-β1 , IL -6 , IL -8 , IL - 10 gene expression to the same person without MSCs treated peripheral blood lymphocytes specimens were used as controls. SD rat dermis transplant Wistar rats wound , bandaged , 2 × 10 ~ 6 Wistar rat MSCs injected into Wistar rats via the tail vein by the same surgical treatment without injection of MSCs Wistar mice as controls . Sterile separation of peripheral blood and spleen lymphocytes of Wistar rats , Wistar rats MSCs and ConA treatment , to ConA alone treatment of Wistar rat lymphocytes as controls . 2 × 10 ~ 6 MSCs in different time points via the tail vein injection to Wistar rats , blood was collected via the tail vein before each injection peripheral blood were determined in CD4, CD8 lymphocyte ratio of total lymphocyte count as a whole . Results : MSCs MSCs ConA-stimulated lymphocyte proliferation and the ratio of lymphocytes , when the ratio is large MSCs ConA-stimulated lymphocyte proliferation significantly inhibited , but the ratio is small , it will promote their proliferation . MSCs on lymphatic
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