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Objective To study the pancreatic tissue of HGF and spleen tissue TGF-β 1 's role in the proliferation of hepatic oval cells (hepaticoval cells, HOC) to explore the impact of endocrine regulation of liver stem cells . Methods weight of 90 , 150-200g male Wistar rats were randomly divided into three groups : control group , AAF / PH group ( hepatic oval cell proliferation ) , 2 - AAF / PH SPL ( splenectomy ) group . To establish hepatic oval cell proliferation model (2-AAF/PH) and 2-AAF/PH SPL animal model . Immunohistochemistry and RT-PCR method to observe the different time points of the three groups of animal models of hepatic oval cell proliferation , the pancreatic HGF and its mRNA expression and spleen of TGF- β 1 , TGF -β 1 mRNA expression changes . Results of a control group in liver resection no oval cell proliferation ; 2-AAF/PH in liver resection first four days of oval cells ( 9.23 ± 4.82 , n = 5 ) , 8 days up to peak (31.72 ± 2.11n = 5), later began to decline , the first 16 days ( 15.80 ± 6.45 , n = 5 ) ; 2-AAF/PH SPL hepatic oval cell proliferation follows four days large numbers of (11.72 ± 0.4, n = 5), eight days reached the peak of proliferation ( 39.23 ± 0.18, n = 5) , and the delay to 12 days ( 37.08 ± 1.87, n = 5) , 16 days down ( 19.71 ± 1.60, n = 5) 2 control group of pancreatic acinar cells weakly expressed HGF ,2-AAF / PH group pancreatic acinar cells four days HGF upregulation (9.4 ± 1.5, n = 5), and eight days reached the peak ( 21.5 ± 7.2 n = 5), 16 days down to normal ( 2.5 ± 0.6 , n = 5 ) . Between the two groups have a significant difference , P lt; 0.05 . Pancreas HGF expression changes in liver HGF expression synchronization , and
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