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Objective: French Mouret completed the first case of laparoscopic cholecystectomy the resection (LC) since endoscopic techniques in general surgery, maternity, bleeding, chest widely used, laparoscopic surgery technology is rapidly around the world to promote, expanding the scope from a single gallbladder resection extended at most subjects, the diagnosis and treatment of the sick species, including many infectious diseases, such as tuberculosis peritonitis, chronic salpingitis, acute cholecystitis, acute pelvic inflammatory disease, suppurative appendicitis, bile peritonitis, severe acute cholangitis, severe pancreatitis. Compared with open surgery, laparoscopic surgery with minimally invasive characteristics, local trauma, systemic response to light, quick recovery of organ function. Made pneumoperitoneum technology is the premise of the majority of laparoscopic surgery, CO 2 is not easy to blasting, solubility in blood and tissues, the formation of air embolism possibility, is commonly used in inflatable gas. Previous studies more CO2 on the body system, and little is known about the impact of pneumoperitoneum on the inflammatory response. Severe abdominal infection prone to toxic shock or multi-organ failure, and even lead to death in patients with laparoscopic techniques applied to the diagnosis and treatment of these diseases, the CO 2 gas abdominal its effect, This is a problem in front of us, we want to provide some answers to this problem parameters through this experiment. Therefore, the discussion the CO 2 gas abdominal severe intra-abdominal inflammation is necessary, it can provide a theoretical basis for the clinical application of laparoscopy in these diseases. Methods: 50 male SD rats, the general level ,10-12 weeks, weighing 300-350g were randomly divided into five groups, each group 10: Ⅴ group, CO , gas the abdominal control group (CO 2 pneumoperitoneum (3 mmHg) for 30 minutes and the line right lower quadrant 1cm incision). IV group, 5cm 1cm small incision and ventral midline laparotomy control group (row right lower quadrant incision). Group III, severe abdominal inflammation group (line cecal ligation and puncture). Ⅱ group, pneumoperitoneum (pneumoperitoneum (3 mmHg) 30 minutes and the line cecal ligation puncture). Group I, the laparotomy group (abdominal median 5cm incision and cecal ligation and puncture). Wichterman cecal ligation and puncture copy of rats with severe inflammation of the abdominal cavity model, laparotomy and pneumoperitoneum after treatment, respectively, after 8 hours, 16 hours, 24-hour pumping peripheral blood determination of serum tumor necrosis factor-alpha (TNF-alpha) , interleukin -6 (IL-6), C-reactive protein (CRP). Results: compared with the control group of CO 2 gas ventral (V-group), the open group (group I), pneumoperitoneum group (group II), severe abdominal inflammation group (group III) serum levels of tumor necrosis factor-alpha (TNF-alpha), interleukin -6 (IL-6), C-reactive protein (CRP) at three time points were significantly higher (p lt; 0.05) With open control group (group IV)
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