|
Objective : To observe the Longhu Qihuang soup of carbon tetrachloride ( the CCl 4 < / sub> ) , D - galactosamine (D-GaIN) due to acute chemical liver injury in mice and Guerin (BCG) lipopolysaccharide (LPS) - induced mice acute liver injury , and to explore its mechanism of action , and the observed toxicity , provided the experimental basis for clinical application and further research . Methods : ( 1) . To Bifendate positive control , with CCl 4 , D - GaIN cause acute chemical liver injury in mice observe the Longhu Qihuang soup high , medium , low dose ( equivalent of crude drug content 30,15 and 7.5g/kg) , respectively . Mice serum and liver tissue homogenates , serum alanine aminotransferase ( ALT ) , aspartate aminotransferase (AST), the tissue homogenates malonyl aldehyde ( MDA ) , superoxide dismutase (SOD), carbon monoxide (NO), α - the content of the tumor necrosis factor (TNF-α) , and mouse liver tissue pathological changes observed in the light microscope . ( 2 ) mice were selected and grouped ibid., mouse Acute liver injury caused by BCG LPS , other steps as above . ( 3 ) . Mice were randomly divided into six groups , with different concentrations of dragon and tiger Qihuang soup gavage , observed 7d deaths . Results: ( 1) dragon and tiger Qihuang soup significant to reduce by CCl 4 induced serum ALT , AST and liver tissue MDA increased significantly improve the content of the liver tissue SOD , can significantly improve the CCl 4 -induced liver histopathological damage , liver tissue nO , TNF -α content had no significant impact . (2 ) Dragon Tiger Qihuang soup can be significantly reduced by D-GaIN induced serum ALT , AST and liver tissue MDA increased significantly improve the SOD content of the liver tissue , can significantly improve due to D-GaIN liver tissue disease Science injury , while TNF -α levels had no significant effect on liver tissue nO . ( 3 ) Longhu Qihuang soup can be significantly reduced by BCG LPS - induced serum ALT , AST and liver tissue MDA rise significantly improve the content of the liver tissue SOD , inhibition of TNF-α in liver tissue increased significantly improve BCG LPS -induced liver histopathological damage , while no significant effect on the NO content of the liver tissue .
|