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Preparation of new bio- electrochemical sensors

Author: ZhaoSuCai
Tutor: WangLi
School: Jiangxi Normal University
Course: Analytical Chemistry
Keywords: Electrochemical sensors Polyethylene ferrocene Thrombin Aptamer Gold nanoparticles Cytochrome c Horseradish peroxidase Glucose oxidase
CLC: TP212.2
Type: Master's thesis
Year: 2010
Downloads: 111
Quote: 0
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Abstract


Known proteins in living organisms many lives in vivo interactions play an important role in the process, for electron transfer between protein has attracted interest of many researchers, some of the artificial signal chain biosensor constructed in a similar to study electron transfer between proteins. On the other hand, protein detection is the focus of today's proteomics research. This thesis is based on hybrid protein modified electrode was studied the transmission of information between proteins and nucleic acid-based aptamer modified electrode was prepared by electrochemical sensors detect thrombin. Specific work is divided into the following four aspects: 1, the first part of the use of aptamer / polyvinyl ferrocene modified electrode preparation of new thrombin sensors. And studied the supporting electrolyte, deposition time, the modified electrode buffer pH detection of thrombin effects. The sensors do not need to mark on the aptamer and the polymeric membrane stability, high sensitivity, response signal strength, and easy operation. 2, the second part of the study of nucleic acid aptamers - Preparation of gold nanoparticles modified electrode electrochemical thrombin sensors. And studied the electrode activation time, thrombin incubation time, incubation temperature, medium impact of the modified electrode. Experiment, the reduction of gold nanoparticles signal to detect thrombin. The thrombin sensor is simple, fast, response signal strength, and can specifically detect thrombin. The sensor further development is expected in real life, the diagnosis and treatment of the disease have been widely used. 3, the third part of the study of the horseradish peroxidase (HRP) and cytochrome c (Cyt.c) mixed protein film modified electrode electron transfer. While these two proteins are not sure whether there is cooperation in vivo, we can in this study to analogy between the electron transport protein. Experimental results show that: Hybrid Protein modified electrodes than a single electron transfer rate constant of electron transfer proteins modified electrode rate constant to be large, which indicates that hybrid protein membrane proteins and enhanced electron transport between the electrodes. The dual enzyme modified electrode has a very good oxygen and hydrogen peroxide catalytic response. 4, the fourth part of the assembly was prepared with layers of GOD-Cyt.c hybrid proteins were modified electrode and its dynamics. The results showed that: Hybrid protein membrane modified electrode surface of the electrode can contribute greatly to the solution of glucose oxidase and an electron transport between, in both cytochrome c oxidase is glucose provides a good biological micro-environment, but also between the electrode and the solution electron transport provides good electron transport channels.

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