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Objective: To observe the expression of Gaq/11 in lung and spleen of rats during acute lung injury （ALI） and effect of propofol treatment, to explore the pathophysiological mechanism of ALI from molecular level. Methods: 48 Wistar rats were randomly divided into experimental groups （OA groups, n=32） given oleic acid from tail vein （OA 0.2ml/kg）, propofol treatment group （P group, n=8） given 1% propofol （80mg/kg/h） from left internal jugular vein for 30 minutes before given the equal dosage OA, and observed for 90 minutes, and control group （C group, n=8） given normal saline （0.2ml/kg）. OA groups included 4 groups: OA30min, OA60min, OA90min, and OA120min. Blood gas （pH, PaO2, PaCO2） and MABP indexes, enzyme indexes （SOD, MDA, NO） of plasma and two organs were measured. Gaq/11 concentration in two organs was examined by Western blot. The structure of spleen was observed by both light microscope and transmission electronmicroscope. Results: PaO2 of OA groups and P group reduced （P<0.01）, and PaO2 of P group was higher than that of OA 90min group （P<0.01）. MABP of OA groups and P group obviously decreased （P<0.05 or 0.01）, and MABP of OA 90min group was higher than that of P group （P<0.01）.SOD activity in plasma and two organs of OA groups （except OA30min group） and P group was lower than that of C group （P<0.05 或 0.01）. SOD activity in plasma and lung of P group was higher than that of OA 90min
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