|
Objective: tongue squamous cell carcinoma (squamous cell carcinoma of the tongue, referred to as tongue cancer ) is the most common malignancy of the oral mucosa , highly malignant, invasive , early metastasis . Treatment is still based on the combined therapy of surgery, chemotherapy , radiotherapy , surgical trauma , put strong side effects of chemotherapy , it is necessary to develop toxicity and good therapeutic effect of new anticancer drugs to reduce mortality of cancer patients and improve their quality of life . In recent years, studies have shown that tanshinone IIA (TanshinoneIIA) of natural medicine by the killing of tumor cells , inhibiting the growth of tumor cells and can induce differentiation and apoptosis , inhibition of invasion and metastasis mechanism play its anti-tumor activity in a variety of tumor cells . However, the of Tanshinone IIA of tongue cancer has not been reported . This article aims to human tongue carcinoma cells by observing Tanshinone IIA the Tca-83 in vitro proliferation , apoptosis situation , to investigate the mechanism of tumor suppression , to provide a theoretical basis for its clinical application . Materials and methods : 1 material : ( 1) cell lines : tongue cancer cell line Tca- 83 were purchased from Peking University, Oral and Maxillofacial Surgery Laboratory . (2) drugs : Tanshinone IIA, Pharmaceutical and Biological Products in China by the reference substance . 2 methods: (1) Solution preparation : Tanshinone IIA with anhydrous ethanol dissolved formulated as a 20mg/mL stock solution was diluted with the culture medium before use . (2) cell culture : in vitro culture Tca-83 passaged cell lines , added with RPMI-1640 medium with 10% fetal bovine serum and the penicillin ( 100U/mL ) , streptomycin (100μg/mL), at 37 ° C , 5 % cultured in an incubator saturated humidity , CO2 , 48 ~ 72h to generation , 0.04% triethylamine tetraethyl
|