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Experimental Study on Osteonecrosis and Bone Defect of Rabbit Femoral Head Treated with Vascular Bundle Implantation Associated with Cellular Transplantation

Author: ChenShuangTao
Tutor: ZhangWeiPing；LiuChangAn
School: Hebei Medical University
Course: Surgery
Keywords: Necrosis of femoral head Liquid nitrogen refrigeration Bone marrow stromal cell BrdU Allotransplantation Vascular bundle implantation
CLC: R687.3
Type: Master's thesis
Year: 2006
Downloads: 62
Quote: 0
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Abstract


Objective:To discuss the feasibility of osteonecrosis and bone defect of the rabbit femoral head treated with vascular bundle implantation associated with allogenic bone marrow stromal cells (BMSCs)transplantation.To explore a new theraphy on treatment of osteonecrosis of femoral head,and to establish the experimental foundation for further clinical application.Methods:1 The preparation for allogenic bone marrow stromal cells transplantation:Firstly,the rabbits’posterior iliac crest were punctured and 3ml marrow was withdrawled,which was inoculated in 6-well dishes.Secondly,5ml conditional DMEM medium (15% fetal bovine serum、100u/ml penicillin sodium、100u/ml streptomycin、10mmol/Lβ-glycerophosphate sodium、10-8mmol/L dexamethasone、50mg/L vitamin C) was mixed into it,and then the 6-well dishes was put into humidified incubator.The medium was replaced for the first one after 5 days,since then,it was replaced every 3 days.Cells of the 3rd generation were kept refrigerated in liquid nitrogen,and were revived after 7 days.When cells of the fifth generation were born, 100μl BrdU was mixed into it,and the telo concentration of

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CLC: > Medicine, health > Surgery > Orthopaedic Surgery ( movement system diseases,orthopedic surgery ) > Orthopedic surgery and surgery > Bone surgery
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