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Objective: Porphyromonas gingivalis Aeromonas blessing Saybolt Bacteroides , spirochetes the three recognized periodontitis important suspicious pathogenic microorganisms . Loesches study confirmed that the the subgingival plaque above three pathogenic microorganisms are able to produce a trypsin-like enzyme (Trypsin-like enzyme, TLE), trypsin-like enzyme is important periodontal tissue destruction of one of the factors . BANA test (bengoyl-DL-arginine-naphthlamide, BANA)) is the use Porphyromonas gingivalis Aeromonas teeth Treponema blessing Saybolt Bacteroides can produce trypsin-like enzyme , allows synthetic enzyme substrate - - benzoyl arginine naphthyl amide (bengoyl-DL-arginine-naphthlamide) hydrolysis of the principle , the establishment of the detection of these three the periodontitis important suspicious pathogenic microorganisms method. In this study, the liquid BANA method reference standard , designed to determine the optimal reaction conditions improved solid phase BANA assay method , to explore improved solid phase BANA test sensitivity , specificity, and the establishment of more convenient in clinical stable solid phase BANA test technical solutions applied to the value of the clinical evaluation of the method to explore the relationship between improved solid phase BANA test results with periodontitis main clinical indicators . Materials and methods 1 bacterial culture test strains selection of Oral important international standard strains 20 , including 14 of anaerobes , aerobes six bacteria were cultured bacterial suspension , respectively, for the solid , liquid BANA trials.
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