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180 birds a day old Cherry Valley ducklings, feeding a week later, were randomly divided into two groups: the test group of 120 birds, the right leg intramuscular new duck hepatitis virus solution 0.2ml / feathers, control group 60 birds, injection of the same amount of the same site. saline solution. At 12 hours after inoculation, 24h, 48h, 72h, 96h, 168h Show details for International Monetary Fund International Monetary Fund. Killed 8 birds each test group and control group, gross anatomical and histopathological examination. While collecting blood and diseased, spectrophotometry serum, liver, brain homogenates catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD ) activity, malondialdehyde (MDA) and nitric oxide (NO) content. The clinical symptoms: after inoculation, 12-24h, ducklings depression, necking arched performance, followed by convulsions, and death was a typical opisthotonos Zhang posture. The time of death is concentrated in the 24-48h after inoculation, the mortality rate was 21.7%. Necropsy shows: significantly enlarged liver, color gray-yellow, brittle texture, surface scattered bleeding and bleeding spots; gallbladder filling; tip gray necrotic pancreatic surface; splenomegaly, the surface was red and white mottled like ; the meninges surface vascular dilatation and congestion. Histopathological changes: swelling of the liver cells round vacuoles of varying sizes in the cytoplasm, severe ballooning degeneration, the hepatic cord structural disorder, inflammatory cell infiltration in the tissue, liver bleeding, liver tissue distribution heterophilic granulocytes, the portal area bile duct hyperplasia. Brain tissue with severe congestive perivascular gap widened small blood vessels around the the lymphoid cells cuff phenomenon, there is phenomenon of neuronophagia yuan, the satellite phenomenon microglial nodules. SOD activity: serum SOD activity 24h after inoculation, rising to the highest value, 96h was significantly lower than the control group, returned to normal after inoculation 7d. Liver SOD activity vaccination after 24 and 48 hours was significantly lower than the control group, other times no significant difference compared with the control group. No significant changes in brain tissue SOD activity throughout the trial period. GSH-Px activity: serum GSH-Px activity after vaccination 48h pole was significantly lower than the control group, 72h was significantly lower than the control group returned to normal after 96 hours. CAT activity: CAT activity in the liver tissue began to decline after inoculation 48h, 96h was significantly lower than the control group, 7d return to normal. MDA content: the content of MDA in serum and liver tissue 24 hours after inoculation extremely significantly higher than that in the control group, no significant change in the content of MDA in brain tissue throughout the trial period. The NO content: the concentration of NO 48h after inoculation began to increase after inoculation continued to 96h, 7d back to normal. NO content in the liver tissue only after inoculation 24h, significantly higher than those in the control group. NO content in brain tissue did not change significantly throughout the trial period. The new ducklings viral hepatitis pathogenesis and pathophysiological changes have not yet fully elucidated, its pathogenesis is very complex, immune and non-immune factors are involved. The experimental results show that, liver disease, the development process,
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