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Edge Anaplasmosis (Anaplasma Marginale Disease) is determined by the edge Anaplasma (A. marginle) caused by a tick-borne infectious blood parasites. The disease mainly caused by cattle, sheep, deer and other livestock disease, can be deadly serious, cattle mortality rate of up to 80%. It is impeding the development of cattle industry in several major tick-borne diseases, the most widely distributed one. The disease has been the International Veterinary Bureau and the Ministry of Agriculture Animal Quarantine as the main target. At present, domestic and foreign has established a number of Anaplasmosis edge detection methods, such as: complement fixation test (CF), the card agglutination test (CAT), competitive ELISA (cELISA), dot-ELISA, indirect fluorescent antibody test (IFAT) , nucleic acid probes, PCR, etc. However, the above method is cumbersome and technically demanding, requires expensive equipment, costly, time consuming, etc., is not conducive to timely immunization against the disease monitoring and diagnosis, thereby limiting their use at the grassroots level to promote. Latex agglutination test (LAT) Due to rapid sensitive, simple, good specificity, etc., in the diagnosis of many diseases have been widely used, but has not been detected by this method the edge of anaplasmosis were reported. In order to study with the LAT detects edges Anaplasmosis, the author through the edge of Anaplasma liquid nitrogen storage insect strains inoculated cattle artificial resuscitation, when the trial worm infected bovine red blood cells peaked, mining anticoagulants, intravenous inoculation Another head test cattle, bovine erythrocyte infection when the test insects rate peaked taken anticoagulant. Then with 0.85% ammonium chloride broken edges Anaplasma infection of red blood cells, centrifugation, sonication parasites obtained latex agglutination test for Anaplasma soluble antigen. Anaplasma the edge soluble antigens by chemical cross-linking approach carboxylated latex sensitization, made of latex antigens. Successfully established a method for detecting the edge of anaplasmosis Bovine serum antibody latex agglutination test (LAT) diagnostic method. Anaplasma antigen using this latex were detected sergenti Theileriosis, Babesia bigemina disease, chlamydia, eperythrozoonosis positive sera were negative; while with the standard positive serum, plasma edge without immune bovine serum, serum samples from endemic areas, showed obvious agglutination. The results show that the edge of developed Anaplasmosis LAT method is particularly suitable grass field edge detection anaplasmosis serum antibody, is simple, rapid, high sensitivity and specificity, low price advantages.
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