Dissertation > Excellent graduate degree dissertation topics show

Cryopreservation System of Pummelo Germplasm Resources

Author: XuDingHua
Tutor: PengZhenKun
School: Huazhong Agricultural University
Course: Pomology
Keywords: Pomelo Germplasm Resources In vitro conservation Cryopreservation
CLC: S666.3
Type: Master's thesis
Year: 2005
Downloads: 147
Quote: 2
Read: Download Dissertation

Abstract

The traditional form of preservation of plant germplasm Planting However, the most vulnerable to the impact of natural climate, space constraints and the shortage of funds and other factors, so that it applied to the long-term preservation of germplasm resources is restricted. In recent years, based on the tissue culture technology developed liquid nitrogen cryopreservation technology for its storage stability, high efficiency, spend less, etc. is considered to be the ideal method for long-term preservation of plant resources. In this study, we in western Hubei unique pummelo germplasm as test materials, Cryopreservation by vitrification, in order to establish the long-term, stable preservation system for long-term preservation of the pummelo resources and other varieties of fruit trees to provide technical draw , the main results are as follows: 1. grapefruit adult stem segments in vitro culture were studied. According to the different characteristics of the fungal spore germination characteristics and different growth stages of sensitivity to disinfectants, August grapefruit adult stem segments as test materials, test to establish an effective sterile explant method, is about 3 ~ 4cm long the grapefruit stem segments with 70% alcohol disinfection 30s, 0.1% mercuric chloride treatment for 20 minutes, the material is placed 2d after the second treatment of the same concentration of mercuric chloride for 20min. Sterile water, the material is cut into 0.5cm long with an axillary bud stem segments were inoculated to the initial medium. In this way, disinfection, explants pollution rate is much lower, the success rate of 85.6%. Grapefruit axillary bud induction medium were screened, the results showed that the the stem segments axillary bud induction optimum medium: the the MT BA 2.0 mg / L GA ₃ 0.5 mg / L NAA 0.1 mg / L glucose 30g / L. When the shoots grow to 1.0 to 1.5cm long the subculture, normal growth, which was not observed in callus phenomenon. 2 grapefruit shoot tips by vitrification cryopreservation basic conditions were studied, initially set up the the pummelo shoot tips by Vitrification Cryopreservation system. Test as a high concentration of sucrose permeability of protective substances, pre-culture in order to the Jingyang Baiyou as test materials. The results showed that pre-incubation of sucrose to 0.3M (1d) 0.5 M (1d) 0.7m (1d) best save a survival rate of 81.4%; vitrification solution (the PVS ₂) dewatering time and temperature, the result of the processing of the local Yichang grapefruit that dehydration was carried out at 0 ° C under 20min, can obtain the preservation of 72.2% survival rate was significantly higher than 20 ° C under processing; stems of the four varieties of grapefruit tip size on the the save Survival of the research results to a size of 2.0 ~ 2.5mm shoot tips of the average survival rate is higher than the addition of both (1.0 to 1.5mm, 3.0 to 3.5mm), was 60.6%. Renewable the plant rooting effective medium formula is: 1/2MT NAA 0.5 mg / L Suc40g / L, the rooting rate reached 90.6%.

Related Dissertations

CLC: > Agricultural Sciences > Gardening > Fruit trees gardening > Citrus > Grapefruit ( pomelo )
© 2012 www.DissertationTopic.Net Mobile