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Swine leukocyte antigen (Swine Leucocyte Antigen, SLA) composite body by the presence on chromosome 7, a group of close linkage and having a composition of a chromosomal region of highly polymorphic genes, coding tissue compatibility antigens. SLA complex region genes of swine breeding for disease resistance candidate genes related studies also show that the SLA complex regional gene of certain blood inventory haplotype pig susceptibility to a variety of diseases and a variety of economic traits related. SLA range of regional gene and most of them are highly polymorphic gene. SLA-DQA gene, the research object, starting from mRNA to light in the United Kingdom, three varieties of Swedish Landrace and Tongcheng pigs by RT-PCR direct sequencing, the clone side sequencing and PCR-RFLP method the entire gene coding DNA polymorphism exists scan and analyze the association between the polymorphism and traits, the following results were obtained: 1, Tongcheng pigs DQA gene coding sequence was cloned by RT-PCR method, sequence alignment after found in the coding sequence of 722 bases at a new SNP loci, the locus bases in the conversion causing amino acids from Gly to Arg transition between A and G, and Taq I restriction sites. By direct sequencing of RT-PCR products of the Large White, Landrace and Tongcheng pigs three varieties DQA gene coding region polymorphisms were analyzed, Large White found that a total of 45 (29P 16S) variable sites : Landrace total of 39 (25P 14S) variable sites; 59 Tongcheng pigs (45P 14S) variable sites. Combined cloning and sequencing, genotyping heterozygous loci, analysis of the RQA gene codon usage frequency, a polymorphic distribution and allele types. Selected in three varieties presence of the polymorphism of the the polymorphic bit 46,169,209,264,274,285,294,299,331,355,372,410 12 sites associated with the part of the immune traits and production traits analysis of results found: a significant level (P lt; 0.05) 46-bit (ACC → GCC) polymorphism with average blood cell volume, slaughter rate related linked, while with the three-point average backfat thickness association is also close to the level (P = 0.063); 169-bit (GAA → CAA) polymorphism with eye muscle area, legs, rump bone rate and other correlation reached significant significant or very significant level; Section 274 (TTA → GCA / GGA) polymorphism related to DTH, IgG, within Lipid rate, loin eye area, suet rate, the ratio of ham, leg rump bone percentage: 285 (AAT → AAC) polymorphism with DTH, within the fat percentage , loin eye area, suet rate the legs hip ratio related; 294 (ATT → ATC) and white blood cell count, IgG content, eye muscle area and muscle tenderness; 299 (ACT → ATT) and DTH ophthalmoplegia the area related: 331 (CAG → AAG) and white blood cell count 6-7 intercostal backfat thickness, muscle tenderness Related: 355 (TCC → CCC) associated DTH, loin eye area; 372 (ATA → ATG) and DTH, within fat percentage, eye muscle area, suet, legs hip ratio related: 410 (AGC → AAC) within the fat percentage, the suet rate, leg-hip ratio, flesh-colored related; 209,264 bits two sites found no correlation with any traits. 4, in my room and through the City Animal Husbandry Bureau jointly built experimental groups, 722 Taq I-RFLP polymorphic trait association analysis found that the groups only detected two genotypes, correlation analysis that among different genotypes, including fat percentage, three-point average backfat thickness, 6-7 intercostal backfat thickness the traits significant difference (P lt; 0.05).
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