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The test of melon amplified RAPD-PCR, DAMD-PCR and SRAP-PCR reaction system optimization , the melon DNA as a template . Screened out of the melon polymorphism analysis of 14 RAPD primers , 9 DAMD-PCR primers 10 SRAP primer portfolio . The use of molecular markers to study the genetic diversity of the the melon germplasm resources of 54 melon germplasm dendrogram drawn according to the data obtained by each marker distribution . At the same time , according to the 54 melon germplasm asked similar analysis of genetic difference matrix , predicted there may be a combination of heterosis . The following results : three markers on melon germplasm polymorphism amplification obtained data applications STATISTICA statistical software to calculate the of germplasm genetic differences between and clustering . 14 RAPD primers material , 9 DAMD cited material , 10 SRAP primer combinations of three kinds of molecular markers of 54 melon germplasm of genetic distance in the 0.03 to 0.53 ( average genetic difference in distance to 0.253 ) , 0.03 to 0.52 ( average to 0.270 ) , 0.08 to 0.79 ( average 0.375 ) , three molecular markers were 54 melon germplasm resources of two categories : Ⅰ , far away from other germplasm ; Ⅱ class can be further divided into 2 subcategories , Ⅱ -1, that is the muskmelon ( cantaloupe and Caigua ) II -2 melon ( textured , patterned and cantaloupe ) . 2 three kinds of markers are exist some with efficient distinction 54 parts melon germplasm of primers was 14 RAPD primers was 5 distinction rate in more than 40% , 9 DAMD primers was 4 distinction rate in more than 30% , 10 SRAP primer combinations four distinguish rate above 50% . Melon taxonomy and breeding studies , will contribute to the the melon germplasm resource efficient identification : melon seeds production , will contribute to the efficient identification of seed authenticity and purity . The three markup analysis showed that : melon ( cantaloupe and melon , textured and textured and non- textured and non- textured , cantaloupe and cantaloupe ) relatively small genetic differences between classes ( thin-skinned and thick-skinned ( network pattern, textured and cantaloupe ) , textured and untextured textured with cantaloupe, textured and cantaloupe ) genetic differences are relatively large . Melon breeding generally germplasm in the class with the group , and the class with groups not pay enough attention , so that the degree of mining and the use of the heterosis in melon breeding breakthrough in the future depend on the class .
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