Dissertation > Excellent graduate degree dissertation topics show

Effect of H2 O2 Pretreatment of Autologous Mesenchymal Stem Cells Transplantation on Ventricular Remodeling After Acute Myocardial Infarction

Author: YuanFeng
Tutor: YuShuDong;ShenZhenYa
School: Suzhou University
Course: Thoracic and Cardiovascular Surgery
Keywords: Oxidation pretreatment Bone marrow mesenchymal stem cells Acute myocardial infarction Ventricular remodeling Paracrine
CLC: R542.22
Type: Master's thesis
Year: 2011
Downloads: 10
Quote: 0
Read: Download Dissertation


Objective: H 2 O 2 pretreatment bone marrow mesenchymal stem cells (mesenchymal stem cells, MSCs), in the acute phase of myocardial infarction after transplantation, and study its acute myocardial infarction ventricular remodeling after impact, and a preliminary study of its mechanism. Methods: In vitro: the third generation of MSCs placed in 24-well plates Petri dish culture, respectively, to 0 μmol / L, 20μmol / L, 50μmol / L, 100μmol / L, 200μmol/LH 2 the O 2 pretreatment MSCs cultured 72h, MSCs supernatant was measured by ELISA of vascular endothelial growth factor (vascular endothelium growth factor, VEGF), hepatocyte growth factor (hepatocyte growth factor, HGF) the concentration;, were given H the 2 O 2 pretreatment of MSCs 0h, 12h, 24h, 36h, 48h for 72h by ELISA MSCs supernatant VEGF, HGF concentration. In vivo testing: 20 healthy Taihu Meishan pigs, attentive catheter with gelfoam anterior descending coronary artery (left anterior descending coronary artery, LAD) the second diagonal branch establishment of the pig acute myocardial infarction model, were randomly divided into four groups: in the MI District injection DMEM interventional group A (control group): myocardial infarction after 24h; B group (oxidized the stem cell supernatants group): 24 hours after MI injection after the H 2 O 2 pretreatment of MSCs in culture supernatants; C group (stem cell group): 24h transplantation after myocardial infarction untreated MSCs; D group (oxidized stem cell group): 24 hours after myocardial infarction transplant after H < sub> 2 O 2 preconditioning MSCs. Each group at different time points were measured by ELISA serum levels of tumor necrosis factor-α (tumor necrosis factor alpha, TNF-α) concentration. Myocardial infarction before 24 hours after myocardial infarction, stem cell transplantation 4w line Powerlab Millar, pressure catheter and magnetic resonance detection of cardiac function index. 4w get pig heart stem cell transplantation the TTC measure myocardial infarct size, immunohistochemical staining to detect non-infarct zone of matrix metalloproteinase-9 (matrix metalloproteinase-9, MMP-9) and inhibit the enzyme -1 (tissue inhibitors of matrix metalloproteinases metalloproteinase-1, TIMP-1) expression of vWF (von Willebrand factor, vWF) immunohistochemistry count neovascularization density. Results: In vitro: different concentrations of H 2 O 2 pretreatment of MSCs, 20μmol / L group, VEGF, HGF concentration compared with 0 μmol / L,, 50 μmol / L, the 100μmol / L, 200μmol / L group was significantly higher (p lt; 0.05); the H 2 O 2 pretreatment of MSCs in different time 24h group VEGF, HGF concentration than 0h, 12h, 36h, 48h group was significantly higher (p lt; 0.05). The concentration of serum TNF-α in vivo test: D and B group the same point in time than the A and group C lower (p lt; 0.05). After transplantation 4w D group and group B compared with group A, left ventricular pressure rise and decline of the maximum rate (dp / dtmax, -dp/dtmax), left ventricular developed pressure (left ventricular developed pressure, LVDP) and end-diastolic wall thickness (end-diastolic wall thickness, EDWT), stroke output (stroke volume, SV) expression was significantly increased (p lt; 0.05), myocardial MMP-9 protein expression and myocardial infarct size is significantly reduced compared with group A (p lt ; 0.05), myocardial expression of TIMP-1 protein expression and infarct peripheral zone capillary density was significantly increased (p lt; 0.05); and D compared with group B, D improved more significantly (p lt; 0.05); C group, these indicators compared with group A, no significant improvement. Conclusion: 1,20 μmol of / LH 2 O 2 the pretreatment MSCs24h can enhance cytokine VEGF, HGF secretion. 2, the acute phase after MI transplant H 2 O 2 pretreatment of MSCs, reducing the level of expression of the proinflammatory cytokines, reducing ventricular matrix remodeling, improve the blood of the infarct border zone supply, thereby inhibiting ventricular remodeling. 3, the acute phase after MI transplantation H 2 O 2 pretreated MSCs significantly improved cardiac function, part of the mechanism may be H 2 O < sub> 2 pretreatment increased secretion of cytokines MSCs.

Related Dissertations

  1. The Value of the Heart-Type Fatty Acid Binding Protein in Early Diagnosis of Acute Myocardial Infarction,R542.22
  2. Platelet parameters in patients before and after acute myocardial infarction,R542.22
  3. The Construction and Evaluation of a Novel Non-viral Gene Transfection System and Its Application in Mesenchymal Stem Cells Gene Recombination,R346
  4. The Research on the Best Time of Bone Marrow Mesenchymal Stem Cell Transplant after Myocardial Infarction,R542.22
  5. Relationship between the Severity of Heart Failure and Alteration of Serum Thyroid Hormone Concentration and Peripheral Blood Leukocyte Count in Acute Myocardial Infarction Patients,R542.22
  6. The Effects of Trophic Effects of Bone Marrow Mesenchymal Stem Cells on Astrocytes Induced by Carbon Monoxide in Culture,R329
  7. BMP2 and VEGF165 Genes Transfected Rat Bone Marrow Mesechymal Stem Cells Simultaneity Expression,R329
  8. Study on Risk Factors and Clinical Characteristics of First Acute Myocardial Infarction in the Young and Old Patients,R542.22
  9. Study on Clinical Value of High-sensitivity Cardiac Troponin T Assay in Myocardial Infarction,R542.22
  10. The Influence of Acute Myocardial Infarction(AMI) Area by Transplanting Bone Marrow Stromal Stem Cells(MSCs) with Different Methods,R542.22
  11. Experimental Investigation of Grafting Pathways of Bone Marrow Stromal Cells into Spinal Cord Injury in Rats.,R651.2
  12. Four-point bending stress stimulation of human bone marrow mesenchymal stem cell proliferation activity,R329
  13. Ultrasound technology assessment dimensional strain in patients with acute myocardial infarction in emergency and delay the clinical value of stenting,R542.22
  14. On the presence or absence of previous angina pectoris in patients with acute myocardial infarction risk factors and clinical characteristics of,R542.22
  15. Heart rate variability and coronary artery disease and prognosis of acute myocardial infarction,R541.4
  16. Underwent emergency surgery and PCI in STEMI patients with cardiogenic shock, the clinical characteristics and prognosis,R542.22
  17. Autologous bone marrow stem cell transplantation in the treatment of heart failure after myocardial infarction term efficacy,R542.22
  18. Bone marrow mesenchymal stem cells in rats with severe acute pancreatitis CD4 ~ CD25 ~ regulatory T cells influence and Integrative Medicine,R576
  19. Bone marrow mesenchymal stem cells in the same kind of heart transplantation on Treg cells in rats,R654.2
  20. The Clinical Study of Human Tissue Urokinase Plasminogen Activator in Patients with Acute Myocardial Infarction,R542.22

CLC: > Medicine, health > Internal Medicine > Heart, blood vessels ( circulatory ) disease > Heart disease > Myocardial diseases > Myocardial infarction
© 2012 www.DissertationTopic.Net  Mobile