Dissertation > Excellent graduate degree dissertation topics show

The Detection of Staphylococcus Aureus in Food Using PCR Method

Author: WangShao
Tutor: LiBo
School: Jilin University
Course: Public Health
Keywords: Food Staphylococcus aureus Polymerase chain reaction ( PCR ) Heat - resistant nuclease
CLC: R155.5
Type: Master's thesis
Year: 2005
Downloads: 414
Quote: 3
Read: Download Dissertation

Abstract


Pathogenic microorganisms of food poisoning caused by contaminated food is a direct result of damage to human health hazards of foodborne poisoning caused by Staphylococcus aureus enterotoxin total bacterial food poisoning in the first place , but there is still a lack of ideal especially rapid method for the detection , so easy to cause the laboratory missed and the delays of the clinical diagnosis . In this study, the method specified by the National Institute of Standards (GB4789.10-2003) and PCR methods of detection of 180 food samples collected from nine areas in Jilin Province , a preliminary understanding of the pollution of the province food in Staphylococcus aureus the two monitoring methods were compared in order to find a more rapid , sensitive , specific , ideal testing methods . The experimental results showed that the PCR method of detecting the positive sample was 45 parts , the positive rate of 25 % , wherein the highest positive rate of fish and cooked food contained within 40%, bags of milk is not detected ; GB detected positive samples is 42 parts , and the positive rate of 23.3% , bulk milk positive rate of up to 37% , bagged milk and vegetables were not detected . Positive PCR assay GB law was no significant difference ( ? 2 = 3.0 , P> 0.05) . PCR method established in this study is designed for the heat-resistant Staphylococcus aureus nuclease gene encoding (nuc gene) a pair of primers , the gene for S. aureus are unique and have a highly conserved , amplification purposes fragment length of 422bp. Create a PCR method in the same time , optimizing the reaction conditions, and the susceptibility test and specificity tests . The test results show that the detection limit of the method 4.32pg of Staphylococcus aureus DNA has good sensitivity ; the test results show that the specificity of detection of the PCR method with Bacillus cereus , Salmonella and other bacteria , Staphylococcus aureus -specific amplification of the target fragment , other bacteria were negative , with good specificity . The PCR method can be completed within 24 hours the GB method takes 4 to 7 days , seen the establishment of Staphylococcus aureus nuc gene PCR method has better sensitivity and specificity , the more quickly and easily than GB .

Related Dissertations

  1. Construction of the Recombinant of HSV-2gD Minotope、 HBsAg、 IL-18 DNA Vaccines and Its Inducing Immune Respons in Mice,R392
  2. Animal products quality safety and security regulation of RFID systems,TS201.6
  3. Application Problems and Construction of HACCP System for Exported Manufacturers of Frozen Convenience Wheaten Food in Jiangsu,F426.82
  4. Study on the Determination and Migration of Harmful Chemicals in Paper Food Packaging Materials,TS206.4
  5. Development Research on Functional Food Ingredien Industry in China,F426.82
  6. The Dynamical Evaluation of Three Methods to Diagnose Mycoplasma Pneumoniae Infection in Children,R725.6
  7. Study on the Determination and Migration of Hazardous Chemicals in Polyethylene Plastic Food Packaging Materials,TS206.4
  8. Drug Susceptibility Testing of Staphylococcus Aureus from Children Impetigo and Typing of Staphylococcus Aureus by Randomly Amplified Polymorphic DNA in Chengdu,R440
  9. Prospect of frozen food rice,F426.82
  10. SJ Food Co., Ltd. in Hangzhou Financial Strategy,F406.72
  11. Y food business management system of multi-,F426.82
  12. Regulation of drugs on the Role of body balance,R96
  13. Anti-tumor Effects of Lupeol at Different Regimens and Its Role in Immune Systems,R730.5
  14. The Study of Food Safety Traceability Logistics Based on Qrcode,TP391.44
  15. Enrichment Regularity of Heavy Metal in the Food Chain of Jinyang Lake Ecosystem,X835
  16. Permanence and Periodic Solutions for an Impulsive Reaction-diffusion N-species Food-chain System,O175
  17. Study on Luohe Technical Supervision Bureau of Food Safety Early Warrning System Based on Neural Network,F203
  18. A Study on the Supervision System of Food Safety in Guangdong Province,F203
  19. Research on the Inhibition Effect of Different Preservatives on Staphylococcus Aureus Rosenbach in Cantonese Cured Meat,TS251.1
  20. Research on Equipment Configuration in Grassroots Agro-food Quality and Safety Laboratories,TS207.7
  21. Legal System Research about Recycling and Disposal of Food Waste of Wuhan,D922.181

CLC: > Medicine, health > Preventive Medicine,Health > Nutrition, hygiene,food hygiene > Food hygiene and food inspection > Food hygiene and inspection
© 2012 www.DissertationTopic.Net  Mobile