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With the deepening worldwide industrialization and the increase in atmospheric pollution, abound in the surrounding environment mutagenic carcinogens on human health threat posed by increasingly serious. Antimutagenic agents from the gene level disease prevention, and strengthen health, contribute to human health and longevity, so its very broad prospects for development and application of by national universal attention. Antimutagenic looking from natural plant active ingredients has become the major topic of today's international life sciences. Inonotus obliquus (Inonotus obliquus or Fuscoporia obliqua) belong to the the Polyporaceae brown lying hole genus Inonotus obliquus water extract can inhibit the growth of mouse sarcoma reported in the literature, in Walker 256 carcinosarcoma cells, human MCF- 7 breast cancer cells in vitro and leukemia P 388 cells in vivo experiments, Inonotus obliquus inotodiol has significant cell inhibitory activity, addition, 3β ,22-dihydroxylanosta-8 ,24-dien-7-one and 3β ,22-dihydroxy-lanosta-7, a mixture of 6 (11) ,24-triene cells has significant inhibitory activity. However, the research of the Inonotus obliquus anti-mutagenic effects have not been reported. The To explore anti Inonotus obliquus mutant active ingredient, the experiment using the Ames test test anti-mutagenic activity, to suppress the mutation rate of activity indicators Inonotus obliquus anti effective parts and the active ingredient in the systematic study. In this study, the first of the Inonotus obliquus alcohol mentioning after the extraction part - petroleum ether extract, ethyl acetate extract, n-butanol extract of Inonotus obliquus polysaccharide the Ames resistant mutation test screening effective parts - petroleum ether extracts and the ethyl acetate extract, and the effective fraction of the separation of the chemical composition and the monomer compound anti mutation test. Experimental results show that: the extraction part (a) Inonotus obliquus alcohol extract, petroleum ether extract and ethyl acetate extract higher inhibition rate in the Ames test, antimutagenic effective parts. (B) using repeated silica gel column chromatography to conduct further separation of the petroleum ether extracts and the ethyl acetate extracts, of eight crystalline compound. According to the physical and chemical properties, as well as 1 H-NMR 13 C-NMR and MS spectral data analysis identified seven known compounds of structure, they are: lanosta- 8,24-dien-3β-ol (lanosterol) (1), lanosta-8 ,24-diene-3β, 22R-diol (inotodiol) (2 and 3), Ergosterol peroxide (5), lanosta-8, 24 - diene-3β, 21-diol (uvariol) (6), 3β-hydroxy-lanosta 8,24-dien-21-oic acid (trametenolic acid) (7), 3β, 22, 25-trihydroxy-lanosta-8-ene
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