|
Objective: To study sodium pyruvate CPB (cardiopulmonary bypass) in the red blood cell energy metabolism and NO . Methods : Healthy adult mongrel dogs ( body weight 12-18kg) 10 Zhi intraperitoneal injection of 2.5% pentobarbital (25mg/kg) anesthesia , femoral arterial puncture , dedicated heparinized catheter and a volumetric flask released canine blood 500ml, 500ml blood is divided into two , were randomly divided into control group ( group C , n = 10 ) and sodium pyruvate (P group , n = 10 ) , the two groups were the dogs blood injected in cardiopulmonary bypass device isolated cardiopulmonary bypass model . Group P pre-filled the sodium pyruvate 250ml , C group with normal saline pre-filled , and constant speed turnaround 4L/min flow , the turnaround after blood temperature maintained in the 30-32 ° C , respectively , before CPB CPB30min , 60min 90min, 120min blood 5ml were detected by red blood cells within the ATP, ADP, AMP concentration , as well as changes in NOS enzyme activity in the plasma concentration of NO and plasma . Results : CPB after the start with the time , compared with before CPB , C RBC ATP , ADP, and AMP concentration was progressively reduce P erythrocyte ADP , AMP CPB30min and 60min higher than before CPB ( p lt; 0.01), but in 90min and 120min gradually reduced ; comparison between groups CPB30min and after each time point P group erythrocyte ATP the AMP concentration higher than the same point in time in group C ( p lt; 0.01 ) ; erythrocyte ADP concentration in CPB30min ( ? ) 190 min higher than group C the same time point (p lt; 0.05), while in the same time point lower than the C group CPB120min . Compared with before CPB , the C group, the plasma concentration of NO and NOS enzyme activity showed increased resistance ; the P group plasma NO concentration and NOS enzyme activity gradually decreased plasma CPB30min and beyond the point P group ; comparison between groups NO concentration decreased , and in CPB90min and 120min difference was statistically significant ( p lt; 0.01 ) ; of NOS enzyme CPB30min ( ? ) 90min below the same point in time in group C ( p lt; 0.01 ) , while in CPB120min higher than that in group C ( p lt; 0.01 ) . Conclusion : CPB can cause red blood cell ATP , ADP , AMP concentration decreased and increased plasma NO concentration and NOS enzyme activity , leading to red blood cell damage and energy metabolism ; sodium pyruvate increased CPB erythrocyte ATP , ADP , AMP concentration and effective regulation of plasma NO content and NOS enzyme activity , better protective effect of CPB in the red blood cell function .
|