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Objective : To observe the allogeneic nonmyeloablative bone marrow transplantation for multiple sclerosis in animal models of disease -EAE rats efficacy and complications , compared with conventional treatment and explore non- myeloablative stem cell transplantation in the treatment of multiple sclerosis The efficacy and safety . Method : First, the establishment of the EAE model , a saline homogenate made ??of complete Freund's adjuvant immune antigen with guinea pig spinal cord , by subcutaneous injection of active immunization of female Wistar rats , 14 days after the guinea pig spinal cord saline homogenate incomplete Freund's adjuvant again immunity induced experimental autoimmune encephalomyelitis occurred . Through observation Wistar rats body weight and symptoms , signs , neurological score to determine the severity of rats occurred EAE and the disease . Bone marrow transplantation in the treatment of EAE rats of the experimental group , compared with the positive control and blank control group concluded . Wistar rats received again 5 days after the immunization , choose the neurological score 2-5 points EAE rats as an animal model of MS , stratified by rating randomized , divided into three groups A, B, C . Group A was the control group , no special treatment ; B group the hormone therapy group , five days after the second vaccination antigen given prednisone orally administered according to the weight 10mg / ( kg · d ) ; C group, bone marrow transplant group , since the second five days after inoculation antigen that six days before transplantation pretreatment, transplant -6, -5 , -4 days after intravenous injection of fludarabine , 1mg / (kg · d ) cyclophosphamide in transplantation -3 , -2, -1 days by tail vein injection of 50 mg / ( kg · d ) . The day of the transplant , according to the weight of 4 × 10 ~ 5 / g , donor mice via the tail vein infusion of bone marrow cells . Healthy male Wistar rats as a bone marrow donor . 5 days after transplantation observe and record the rats in each group after treatment, body weight and neurological function score , continuous observation for 40 days , all rats were sacrificed 40 days after transplantation , the conventional formaldehyde
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