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Relationship between Epithelial-mesenchymal Transition Induced by Hypoxia and Invasive Ability in Hepatocellular Carcinoma Cells

Author: CaoWenZuo
Tutor: WuHaoRong
School: Suzhou University
Course: General surgery Sciences
Keywords: Hypoxia Epithelial - mesenchymal transition Cell migration Cell invasion
CLC: R735.7
Type: Master's thesis
Year: 2011
Downloads: 57
Quote: 0
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Abstract


Objective: To investigate the presence of epithelial - mesenchymal transition ( Epithelial - Mesenchymal Transition , EMT ) , and the resulting liver cancer cell migration and invasion force change, to provide new ideas for the treatment of liver cancer induced by hypoxia in hepatoma cells . Methods: two different metastatic ability of HCC cells by hypoxia establish human hepatoma cell physical hypoxia model , inverted microscope observation of liver cancer cells under hypoxic conditions , morphological changes , the application was detected by Western Blot epithelial cell marker protein E -Cadherin and stromal cell marker protein Vimentin expression changes detected by the image analysis software gray value of protein and protein relative value detected using Transwell chamber after hypoxia liver cancer cell migration , invasion ability . Results: observed under an inverted microscope , cells from hypoxia closely packed state into a loose spindle -like changes in the cells loose . Western blot analysis showed that the epithelial cell marker protein E-Cadherin expression values ??than normal decreased significantly (P lt ; 0.01 ), and stromal cell marker protein Vimentin expression value was significantly higher ( P lt; 0.01 ) . Transwell migration and invasion assay showed that liver cancer hypoxia , its ability to significantly enhance migration and invasion . Hepatoma cells of two different transfer capabilities compared with each other , more obvious change lower metastatic cells highly metastatic cells to hypoxia . Conclusion: hypoxic environment can lead to liver cancer cell epithelial - mesenchymal transition , and enhance the ability of cell migration and invasion , causing liver cancer cells to distant metastasis , and transfer ability of the cells affected by the lack of oxygen is more obvious .

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CLC: > Medicine, health > Oncology > Gastrointestinal Cancer > Liver tumors
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