Dissertation > Excellent graduate degree dissertation topics show

Impact of Bosentan and Amlodipine on Fibrosis and Microvasculatures in the Kidney of DOCA-salt Hypertensive Rats

Author: WangKaiXuan
Tutor: HongXiaoSu
School: Suzhou University
Course: Department of Cardiology
Keywords: DOCA Angiogenesis kidney Hypertension Transforming growth factor β 1 Smad7 Bosentan Amlodipine Endothelin-1
CLC: R96
Type: Master's thesis
Year: 2005
Downloads: 61
Quote: 0
Read: Download Dissertation

Abstract


Objectives: This study was performed to investigate the impact of bosentan and amlodipin on fibrosis and microvasculatures in the kidney of DOCA-salt hypertensive rats（DHR）.Methods: 24 male Sprague-Dawley rats were uninephrectomized ,given penicillin three days.Then randomly divided into 4 groups.,One group served as control group,drinking tap water.Three other groups were administered DOCA subcutaneously （50mg/kg week）and 1%saline was added to their tap water for drinking. they were treated for 5 weeks with either a long-acting calcium antagonist, amlodipine(20mg/kg day-1), mixed ETA-ETB endothelin receptor antagonist,bosentan(100mg/kg day-1).or given placebo. At the end of treatment,systolic blood pressure（SBP）,24 hour urinary protein excretion rate（24h-UPER）,serum blood urea nitrogen（BUN）, creatinine（Scr） were measured. Renal histology change was assessed on PAS-stained sections. The protein expressions of renal transforming growth factor（TGF- β 1） and Smad7 were determined by immunohistochemistry at 5th week.The number of capillary density and proliferating endothelial cells were also counted. Results: DHR exhibited an enhanced 24h-UPER,increased renal histology change ,over-expressions of TGF- β 1 and Smad7 were observed in untreatedDHR compared with control group.All these abnormalities were ameliorated by bosentan, except amlodipine..However, 3UN and Scr remained nomal. The glomerular capillary index（GCI） and peritubular capillary index（PCI） were significantly lower in DHR group than those in control group（P<0.01）.The GCI and PCI in antihypertension drug-treated group were significantly higher than those in placebo group,but the GCI and PCI in bosentan were significantly higher than those in amlodipine-treated group（P<0.05）.The numbers of glomerular and interstitial proliferating endothelial cells in all antihypertensive drug-treated groups were significantly higher than those in placebo group（P<0.01）, but the numbers of proliferating endothelial cells in bosentan group were significantly higher than those in amlodipine-treated group（P<0.05）.Conclusion: Bosentan significantly attenuate renal damage, improves angiogenesis and increases capillary density in DHR,suggesting that endothelin-1 may contribute to progression of renal damage via increases the expression of TGF- ￡ 1 and Smad7,direct inhibition of angiogenesis and indirect damage of microvasculature through exacerbation of hypertension.

Related Dissertations

  1. Study on Relationship between Endothelin-1, Insulin Resistance and Acute Ischemic Cerebrovascular Disease,R743.3
  2. The Correlation Study between Entothelin-1 Level in Blood Plasma and Hemorrheology Changes in Primary Glaucoma and Visual Function Damage Degree.,R775
  3. The Study of Fasudil Hydrochloride Prevented Acute Kidney Injury Rat Caused by Lipopolysaccharide,R965
  4. Endothelin-1 in rat model of aldosteronism role in the process of renal fibrosis and its significance,R586.24
  5. Genetic Polymorphisms of Rage, ECE-1b and TIMP2 and Susceptibility to Gastric Cancer,R735.2
  6. The Study of Using Bosental Treal Acute Lung Injury Induced by Paraquat Poisoning in Rats,R595.4
  7. The Outcome of Dual Endothelin Receptor Antagonist in the Infant Younger Than 6 Months with Congenital Heart Disease (Shunt) Associated Pulmonary Arterial Hypertension,R726.5
  8. Analysis of Correlation of Serum High Sensitive C-Reactive Protein, Brain Natriuretic Peptide, Endothelin-1 with Nonvalvular Atrial Fibrillation,R541.75
  9. Construction and Function Study of the GlnA/ECE1 Gene Knock-Out Mutant of Streptococcus Suis Serotype 2 and Preparation of Monoclonal Antibody Against ECE1,S852.611
  10. Expressions of Endothelin Receptors and Endothelin Converting Enzyme-1 in Pulmonary Hemorrhage in Newborn Rats,R722.1
  11. Effects of Different Medicines on the Induced Nitricoxide Synthase and Endothelin (ET)、Renal Cell Apoptosis in Septic Shock Rats,R459.7
  12. The Effect of Calycosin for ET-1/NO on the Damage of TNF-α in Human Umbilical Vein Endothelial Cells,R285
  13. Clinical Significance of Serum Hypoxia Inducible Factor-1α、Vascular Endothelial Growth Factor and Endothelin-1 in the Patients with Acute Coronary Syndrome,R541.4
  14. Effectiveness of Integrative Therapy with Priority to Acupuncture on Plasma Endothelin in Sequelae of Peripheral Facial Paralysis,R246.6
  15. Comparison Research of Uterine Recovery of Rats after Medicine Abortion in the Midtrimester of Pregnancy by Principles of Regulating Liver to Stop Bleeding, Activating Blood to Expel Stasis and Tonifying Qi to Control Bleeding,R271.9
  16. Bing Huang Wuling liquid proctoclysis treatment of acute cerebral hemorrhage,R277.7
  17. The Liver Modulation and Hemostasis Method Affecting the Uterus Endothelin-1, Nitrogen Monoxidum and Nitricoxide Synthase of the Pregnant Rat after Drug Abortion,R285.5
  18. The Study of Fuming Tablets’ Contribution and Mechanism for Optic Function Recovery via Inhibiting the Proliferation after Rabbit Retinal Detachment,R285.5
  19. Study on the Synthesis of Bosentan, an Antagonist of Endothelion Receptor,R914
  20. The Relationship between the Change of ET-1/SCF in Epidermal Microenvironment of Vitiligo and the Therapeutic Effect of Autologous Epidermal Grafting,R758.41
  21. Clinical Significance of Measuring the CEA and ET-1 in Exhaled Breath Condensate of Patients with Non-small Cell Lung Cancer,R734.2

CLC: > Medicine, health > Pharmacy > Pharmacology
© 2012 www.DissertationTopic.Net  Mobile